DLS Molecular Weight Determination Service

The DLS molecular weight determination service uses dynamic light scattering technology to measure the size distribution and molecular weight of particles in solution, providing critical data for the stability, aggregation, and characteristics of biomolecules and nanoparticles. DLS (Dynamic Light Scattering) does not require labeling of samples, making it suitable for rapid measurement of low-concentration samples. It is widely applied in the analysis of biomacromolecules, natural products, and metabolites. Its label-free, fast, and precise nature makes it an essential tool in protein drugs, vaccines, and nanotechnology. Compared to traditional methods, DLS provides efficient and real-time molecular weight estimation without complex procedures or long experimental times, making it particularly suitable for high-throughput screening and dynamic behavior analysis of particles in solution. Using DLS to measure molecular weight is of significant importance for ensuring the stability of biopharmaceuticals and monitoring drug aggregation. DLS has also become a key technology in life science research and drug quality control.

 

Service at MtoZ Biolabs

MtoZ Biolabs offers precise DLS molecular weight determination services with advanced DLS technology and state-of-the-art laboratory equipment. Using the latest generation of DLS instruments, we efficiently analyze the Brownian motion of particles in solution to accurately measure molecular size and molecular weight. Through our services, clients can quickly obtain molecular weight data, optimizing product development and quality control processes. This service is particularly suitable for drug development, vaccine production, and other fields, helping researchers gain deeper insights into molecular structures and enhance the stability of biopharmaceuticals. MtoZ Biolabs is committed to providing high-quality, reliable molecular weight analysis, supporting innovation and development in both scientific research and industrial applications. If you are interested in our services, please feel free to contact us, and we will be happy to assist you.

 

 

Figure 1. The Principle of Dynamic Light Scattering

Hassan, PA. et al. Langmuir. 2015.

 

Service Advantages

• non invasive instrument

• Fast and Accurate Molecular Weight Determination

• Requires only small quantities of sample

• Good for assessing homogeneity of the sample

• Good for detecting trace amounts of aggregates

• Customized Service Solutions

• Continuous Technological Updates and Innovation

 

Sample Submission Suggestions

1. Sample Concentration  

The sample concentration needs to be within an appropriate range. Too high a concentration may lead to an excessively strong signal, affecting the accuracy of the measurement; too low a concentration may result in a weak signal, making it difficult to obtain valid data. Generally, the concentration should be maintained within the optimal working range of the DLS instrument.

 

2. Sample Purity  

To ensure accurate measurements, the sample should be as pure as possible. The presence of impurities, aggregates, or particles may interfere with the measurement results. If necessary, filtering or centrifugation can be performed to remove larger particles and precipitates.

 

3. Sample Homogeneity  

The sample should be uniformly distributed to ensure reliable measurement results. Prior to the experiment, avoid vigorous stirring or ultrasonic treatment, as these may cause molecular aggregation or sample heterogeneity.

 

4. Solution Conditions  

The pH, salt concentration, and temperature of the solution significantly affect molecular motion and light scattering signals. Therefore, it is crucial to ensure stable solution conditions before and during the experiment. Typically, an appropriate buffer solution should be chosen, and the experimental environment temperature should be controlled.

 

Case Study

1. DLS Determines Molecular Weight of 2', 5'-Oligoadenylate Synthetase

 



Deo, S, et al. PLoS One. 2014.

 

FAQ

1. How to avoid the impact of sample aggregation on measurement results?

To avoid sample aggregation, it is important to ensure that the sample is not overly stirred or improperly stored during preparation, as these factors can lead to molecular aggregation. Before measurement, large particles can be removed by centrifugation or sonication, and appropriate buffer solutions should be used to reduce the formation of aggregates. If aggregation is observed, adjusting the pH, salt concentration, or adding stabilizers can help improve the sample's uniformity.

 

2. How to determine the appropriate sample concentration?  

When determining the appropriate sample concentration, it is necessary to adjust based on the optimal working range of the DLS instrument. High concentrations can cause the scattering signal to be too strong, affecting data accuracy, so the sample should typically be diluted to an appropriate concentration range. Before the experiment, it is recommended to conduct concentration gradient tests to find the optimal concentration point. Additionally, it is important to ensure that the particles in the solution are evenly distributed to avoid data discrepancies caused by either too high or too low concentrations.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Molecular Weight Determination 

5. Mass Spectrometry Image

6. Raw Data