dTMP Analysis Service
- Expert team with extensive experience in metabolomics analysis.
- Advanced technology platform ensuring high sensitivity and accuracy.
- Comprehensive data report providing in-depth analysis.
- Excellent customer service to assist you throughout the project.
- Oncology: Understanding the role of dTMP metabolism in cancer.
- Drug Metabolism: Studying the effect of drugs on dTMP levels.
- Genomics: Investigating the role of dTMP in DNA replication and repair.
- Samples should be stored at -80℃ and shipped with dry ice.
- We recommend providing at least 3 samples of similar conditions for each type of sample.
- Other sample types, please contact us for more details.
Deoxythymidine monophosphate (dTMP) is a nucleotide that plays a critical role in DNA synthesis and repair. dTMP consists of a phosphate group, the pentose sugar deoxyribose, and the nucleobase thymine. It is an intermediate in the thymidine salvage pathway, where it is synthesized from deoxyuridine monophosphate (dUMP) through the action of the enzyme thymidylate synthase. The importance of dTMP lies in its contribution to maintaining the balance of nucleotides necessary for proper DNA replication and repair, which are vital for cell survival and proliferation.
Figure 1. Partial Schematic Diagram of De Novo dTMP Synthesis
Research on dTMP has been extensive, particularly in the context of cancer biology and chemotherapy. Aberrations in dTMP biosynthesis are associated with cellular dysfunctions, including the development of certain cancers. Targeting the dTMP biosynthesis pathway has been a strategy in the design of chemotherapeutic agents such as 5-fluorouracil (5-FU), which inhibits thymidylate synthase, leading to a reduction in dTMP levels and consequently impairing DNA synthesis in rapidly dividing tumor cells. The study of dTMP is also crucial in understanding metabolic disorders and the mechanisms of antiviral drugs that target DNA polymerases. Accurate and detailed analysis of dTMP levels and its metabolic pathways provides insights into the molecular underpinnings of these diseases and aids in the development of therapeutic interventions.
MtoZ Biolabs offers comprehensive dTMP analysis service. We utilize high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) for accurate quantitation of dTMP in a variety of samples. Our experienced team can help you uncover the complexities of dTMP metabolism in your biological system of interest.
Analysis Workflow
1. Sample Preparation: We accept a wide range of biological samples including cells, tissues, and biofluids.
2. Sample Extraction: We extract dTMP from your samples using validated protocols to ensure high recovery.
3. dTMP Analysis: We use HPLC-MS/MS for accurate and sensitive dTMP quantitation.
4. Data Analysis and Report: We provide a comprehensive data report including method validation, data interpretation, and statistical analysis.
Service Advantages
Applications
Our dTMP analysis service can be applied in various research areas including:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on dTMP
4. Raw Data
5. Custom Analysis Report
For more information about our dTMP analysis service, please do not hesitate to contact us. We are committed to providing you with the highest quality data to meet your research needs.
How to order?
