Edman Degradation

Edman degradation is a classic protein sequencing technique specifically used for deducing the amino acid sequence of proteins or peptides from the N-terminus step by step. This method is suitable for purified proteins or peptides and provides accurate sequence information, particularly ideal for analyzing proteins of small to medium size. At MtoZ Biolabs, we offer high-standard Edman degradation services to help researchers precisely identify protein sequences, thereby advancing fundamental biological research and biopharmaceutical development.

 

Technical Principles

Edman degradation involves a cyclic chemical reaction that sequentially removes amino acids from the N-terminus of a protein. After each amino acid is cleaved, it is identified through specific chemical labeling and subsequent chromatographic analysis. This process is repeated until the desired length of the amino acid sequence is obtained or the technical sequencing limit is reached (typically up to 50 amino acids). The advantages of this technique lie in its high specificity and reproducibility, making it very suitable for structural analysis and quality control of biologics.

 



Figure1. Edman Sequencing Workflow

 

Applications

Our Edman Degradation service could be applied in various research fields, including but not limited to:

• Protein Identification
• Antibody Sequencing
• Vaccine Development
• Drug Discovery

 

Sample Requirements

To achieve the best results, we kindly ask you to provide sufficient amount and high quality of samples. For specific sample requirements, please contact our customer service.

 

Choosing MtoZ Biolabs' Edman degradation service ensures precise and reliable protein sequence analysis, supporting your research and product development projects.