Endogenous Fluorescence Detection of Recombinant Protein Drugs

Recombinant protein drugs are protein-based therapeutic drugs which are produced by using DNA recombination technology or other biotechnological methods. These drugs include cytokines, peptide hormones, recombinant enzymes, monoclonal antibodies, and fusion proteins. Compared to traditional low-molecular-weight synthetic drugs, recombinant protein drugs have advantages such as high specificity, low toxicity, and significant therapeutic effects. Additionally, recombinant protein drugs can be used in combination with small molecule drugs to provide additional or synergistic benefits.

 

The intrinsic fluorescence of proteins mainly originates from the aromatic amino acids in their structure, such as tryptophan, tyrosine, and phenylalanine, which emit fluorescence under ultraviolet light. When the protein structure changes, such as folding, unfolding, or binding to ligands, its intrinsic fluorescence also changes accordingly. Therefore, by measuring the intrinsic fluorescence of recombinant protein drugs, one can obtain information about their structure and monitor possible denaturation during the preparation and storage processes, thus assessing the stability of recombinant protein drugs.

 

The detection of intrinsic fluorescence in recombinant protein drugs is typically performed by using a fluorescence spectrometer, which can accurately measure the intensity and wavelength of protein fluorescence to obtain the fluorescence spectrum. By analyzing the fluorescence spectrum, one can gain valuable information about the recombinant protein drugs. Although intrinsic fluorescence detection is a powerful tool, it has some limitations, such as potential interference from quenchers or signal overlap when the sample contains large amounts of aromatic amino acids. Therefore, intrinsic fluorescence detection is often combined with other analytical methods (such as circular dichroism, X-ray crystallography, or nuclear magnetic resonance) to obtain more comprehensive and accurate information.

 

We offer professional intrinsic fluorescence detection services for recombinant protein drugs. In addition to providing structural information and evaluating drug stability, intrinsic fluorescence detection can also detect interactions between recombinant protein drugs and small molecules or other proteins. Furthermore, MtoZ Biolabs also offers a variety of biopharmaceutical characterization services, such as circular dichroism, X-ray, and nuclear magnetic resonance analysis, please feel free to inquire in detail.