Explore Comprehensive Phosphoproteomics with DIA Technology at MtoZ Biolabs

In the dynamic world of proteomics, phosphoproteomics has become an essential area of research due to the critical role phosphorylation plays in regulating cellular processes. At MtoZ Biolabs, we utilize cutting-edge Data-Independent Acquisition (DIA) technology combined with our extensive expertise in proteomics and mass spectrometry to offer a comprehensive phosphoproteomics analysis service. This service helps researchers uncover novel insights into phosphorylation events, signal transduction pathways, and the mechanisms behind various diseases.

 

Workflow of DIA-Based Phosphoproteomics

Our DIA-based phosphoproteomics workflow ensures high-quality and reliable results with unparalleled coverage. The process begins with phosphopeptide enrichment using Immobilized Metal Affinity Chromatography (IMAC), which significantly improves detection efficiency. The DIA technique provides exhaustive and unbiased data acquisition by analyzing all ions within a specific mass range in each cycle, enabling the identification of thousands of phosphopeptides in a single run.

 



 

Instrument Platform Features

At MtoZ Biolabs, we rely on state-of-the-art mass spectrometry instruments, ensuring the highest quality and precision in every analysis. Our platform includes:

1. Q Exactive HF-X (Thermo Fisher Scientific): With ultra-high resolution and mass accuracy, ideal for large-scale phosphoproteomics studies

2. Orbitrap Exploris 480 (Thermo Fisher Scientific): Combines high resolution and fast scanning speeds, ensuring a broad dynamic range and deep phosphoproteome coverage.

3. timsTOF Pro (Bruker): Utilizes trapped ion mobility spectrometry (TIMS) for enhanced ion separation, maximizing sensitivity and reducing analysis time.

 



 

Each instrument is tailored for high-throughput and large-scale phosphoproteomics, guaranteeing excellent performance and consistent results.

 

Quality Features: Multi-Level QC to Ensure High-Quality Data

We prioritize quality control at every step of the analysis, integrating advanced QC systems to ensure accuracy, reproducibility, and reliability. Our QC features include:

▶️Chromatographic Evaluation

The separation effect of the chromatographic peak and the effective chromatographic peak data points reflect the accuracy and stability of the protein quantitative results. The chromatographic effect is evaluated by the average data points of the chromatographic peak, the stability of the internal standard peptide iRT retention time and the peak capacity.

 

▶️QC Evaluation

QC samples are the most effective method for overall data evaluation. The consistency of QC samples is evaluated using quantitative deviation (CV value), principal component analysis, correlation and other methods to reflect the stability and parallelism of the entire data collection process.

 

▶️Sample Evaluation

The samples can be evaluated by testing the ion intensity, PCA distribution and other conditions of the samples.

 

MtoZ Biolabs provides a comprehensive DIA-based phosphoproteomics service that leverages cutting-edge technology, a robust instrument platform, and a stringent quality control system. By partnering with us, researchers gain access to deep phosphoproteomic coverage, high reproducibility, and unmatched precision, accelerating discoveries in cellular signaling, disease mechanisms, and therapeutic interventions. Whether you are conducting basic research or drug development, our phosphoproteomics solutions are designed to meet your needs.