Exploring Quantitative Analysis in Proteomics

Proteomics quantitative analysis, as an efficient and accurate technology, has revealed the depth and breadth of the proteome, providing important support for biomedical research.

 

Principle of Proteomics Quantitative Analysis

Proteomics quantitative analysis is a method that reveals the level and changes of protein expression in organisms by measuring the quantity variation of proteins under different conditions. Commonly used quantitative methods include mass spectrometry quantitative techniques, isotope labeling-based quantitative techniques, and peptide labeling-based quantitative techniques. Among them, mass spectrometry quantitative techniques are widely used in proteomics research due to their high sensitivity, accuracy, and throughput.

 

Methods of Proteomics Quantitative Analysis

1. Mass Spectrometry Quantitative Techniques

These include Multiple Reaction Monitoring (MRM), Quantitative Proteomics (SILAC, iTRAQ and TMT, etc.), and Proteome Analysis (DIA), etc. These methods quantify protein samples through mass spectrometry instruments and can obtain relative or absolute expression levels of proteins.

 

2. Isotope Labeling-Based Quantitative Techniques

These include protein internal labeling, chemical labeling, and metabolic labeling, etc. These methods introduce isotopic labels into protein samples and quantify the expression level of target proteins by comparing the abundance difference of isotopic labels.

 

3. Peptide Labeling-Based Quantitative Techniques

These include peptide segment quantification after protein digestion, Selective Reaction Monitoring (SRM), etc. These methods quantify protein expression levels by analyzing the abundance changes of specific peptide segments produced by protein digestion.

 

Applications of Proteomics Quantitative Analysis

1. Discovery of Disease Biomarkers

Proteomics quantitative analysis can be used to screen potential disease markers, assisting in early disease diagnosis and predicting disease progression.

 

2. Drug Research and Evaluation

Proteomics quantitative analysis can be used to assess the efficacy and safety of drugs, aiding drug development and optimization.

 

3. Disease Mechanism Research

Through proteomics quantitative analysis, we can understand the changes in the proteome under different disease states, revealing disease occurrence and progression mechanisms.

 

Proteomics quantitative analysis, as a powerful technology, has propelled the development of proteomics research. By exploring the depth and breadth of the proteome, we can better understand the function and changes of proteins within organisms, providing an important basis for disease diagnosis and treatment. With the continuous advancement of technology, the application of proteomics quantitative analysis in biomedical research will become more widespread, which is expected to bring new breakthroughs in the discovery and development of new drugs.