Fatty Acid Degradation Analysis Service
- Basic biological research: understanding the role of fatty acids in cellular processes.
- Disease research: studying the alteration of fatty acid metabolism in diseases such as cancer, diabetes, and cardiovascular diseases.
- Drug discovery: evaluating the effects of drugs on fatty acid metabolism.
- Type of samples: Plasma, serum, cell, tissue, etc.
- Quantity: At least 200 μl liquid, 1 x 107 cells, or 200 mg tissue.
- Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice to ensure the stability of fatty acids.
Fatty acid degradation is a critical metabolic pathway that breaks down fatty acids to generate energy. This process primarily occurs through β-oxidation, a series of enzymatic reactions in the mitochondria (and in some cases, peroxisomes), where fatty acids are sequentially shortened by two-carbon units, producing acetyl-CoA. The acetyl-CoA generated enters the citric acid cycle, contributing to the production of ATP, which is essential for various cellular functions. Fatty acid degradation is vital for maintaining energy homeostasis, especially during periods of fasting, prolonged exercise, or when carbohydrate intake is limited.
Beyond energy production, the degradation of fatty acids plays a crucial role in lipid homeostasis, preventing the accumulation of fatty acids and triglycerides that can lead to metabolic disorders. Dysregulation of fatty acid degradation is associated with several health conditions, including fatty liver disease, obesity, diabetes, and cardiovascular diseases. Additionally, specific genetic disorders affecting enzymes involved in β-oxidation, such as medium-chain acyl-CoA dehydrogenase deficiency (MCADD), highlight the importance of this pathway in human health. Understanding the mechanisms and regulation of fatty acid degradation is therefore essential for developing therapeutic strategies and managing metabolic diseases.
Services at MtoZ Biolabs
At MtoZ Biolabs, our fatty acid degradation analysis service is based on high-performance liquid chromatography-mass spectrometry (HPLC-MS). The fatty acids in the samples are first extracted and derivatized, then separated by HPLC, and finally detected and quantified by MS. The data obtained is processed using a sophisticated bioinformatics pipeline for identification and quantification of fatty acids and their metabolites.
Analysis Workflow
Applications
Our fatty acid degradation analysis service can be used in a variety of research fields, including but not limited to:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Fatty Acid Degradation Analysis
4. Mass Spectrometry Images
5. Raw Data
For more details about our Fatty Acid Degradation Analysis Service, please feel free to contact us.
How to order?
