Flavones Analysis Service | UPLC-MRM/MS
Flavones are bioactive substances widely found in plants, with biological activities such as antioxidant, anti-inflammatory, anti-tumor, antibacterial, and antiviral. Most flavones are fixed on the cell walls of plants, and only a few are dissolved in cell sap. The main source of flavones is plants, including vegetables, fruits, nuts, seeds, stems, roots, flowers, tea, wine, olive oil, etc.
MtoZ Biolabs offers a comprehensive Flavones Analysis Service utilizing ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) technology. Our analytical approach employs multiple reaction monitoring (MRM) within the mass spectrometry process to achieve precise qualitative and quantitative analysis of flavones.
Technical Principles
MRM, using reference standards, enables targeted detection and absolute quantification of specific metabolites with high specificity, sensitivity, and accuracy. By selectively acquiring mass spectrometric signals based on known or predicted ion transitions, MRM filters out non-target ions and focuses on detecting only the relevant MS/MS2 ions. This process enhances the precision and reliability of the analysis, providing a highly accurate and sensitive method for metabolite quantification.
Figure 1. Schematic Diagram of MRM Principle
Analysis Workflow
1. Sample Pretreatment: The flavones in the sample are extracted, purified, and concentrated to obtain a sample that can directly enter the UPLC-MS system.
2. UPLC-MS/MS Analysis: UPLC-MS/MS is used to efficiently separate and accurately detect samples.
3. Data Processing: Professional data processing software is used for bioinformatics analysis to obtain accurate flavone quantitation results.
4. Result Interpretation: Professional interpretation of the analysis results, providing comprehensive flavone analysis reports.
Service Advantages
1. High Sensitivity: Using UPLC-MS/MS technology, it has high sensitivity and can analyze flavones in complex biological samples.
2. High Accuracy: Using professional data processing software to achieve accurate flavone quantization.
3. Professional Technical Team: Our technical team has rich experience in flavone analysis and can provide professional technical support.
Applications
Flavones analysis services are widely used in food, medicine, cosmetics, agricultural products, etc., can be used to study the biological activity and pharmacological effects of flavones, and can also be used for product quality control.
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Flavones
4. Mass Spectrometry Images
5. Raw Data
How to order?
