Gel Permeation Chromatography for Molecular Weight Determination Service

Gel Permeation Chromatography For Molecular Weight Determination Service utilizes high-precision gel permeation chromatography (GPC) to analyze the molecular weight and distribution of samples, offering customized testing solutions and multi-detector coupling technologies. It accurately calculates both number-average and weight-average molecular weights, supporting material research, product development, and quality control. GPC separates molecules based on their size distribution within the gel column, with larger molecules eluting earlier and smaller ones later. By measuring the elution time and comparing it with those of standard samples, the molecular weight and distribution of the sample can be determined. Compared to traditional methods, GPC offers higher resolution and broader applicability, effectively analyzing the molecular weight characteristics of macromolecules, supporting research and development in the biopharmaceutical field. Its non-destructive and rapid analysis makes it suitable for different sample forms, offering significant scientific value and practical relevance.

 



Figure1. The Principle of Gel Permeation Chromatography

 

Service at MtoZ Biolabs

MtoZ Biolabs' Gel Permeation Chromatography for Molecular Weight Determination Service, equipped with industry-leading GPC instruments and multi-detector coupling technologies, provides high-precision molecular weight analysis. Our instruments, combined with advanced gel column separation technology, accurately differentiate the various molecules in the sample and quickly calculate its molecular weight, ensuring high accuracy and resolution in the analysis. With years of technical expertise, MtoZ Biolabs has accumulated extensive experience in molecular weight determination, offering customized testing solutions for a wide range of macromolecular and small-molecule compounds to meet diverse research and development needs. If you are interested in our services, please feel free to contact us, and we will be happy to assist you.

 

Service Advantages

1. High-Precision Analysis: Utilizing high-resolution GPC systems, we provide accurate molecular weight and molecular weight distribution data, ensuring precision and reliability in the results.

 

2. Versatile Analysis Capability: Suitable for a wide range of macromolecular samples, including proteins, polymers, and other biomolecules, meeting the needs of various research fields.

 

3. Experienced Team: With years of expertise, MtoZ Biolabs offers customized solutions, optimizing analytical conditions to ensure the high reproducibility of the data.

 

4. Efficient and Fast Service: Through advanced GPC technology and efficient experimental workflows, we can provide detailed and accurate molecular weight analysis reports in a timely manner, supporting customers to quickly advance their research and development projects.

 

Sample Submission Suggestions

1. Sample Purity: The submitted sample should be a solution of high purity, with minimal impurities or contaminants. Low purity samples may affect the accuracy of molecular weight determination results.

 

2. Sample Concentration: The sample concentration should be moderate. High concentrations may cause overlap of chromatographic peaks or distortion of peak shapes, while low concentrations may affect sensitivity and the accuracy of the data.

 

3. Sample Preparation: Ensure that the sample is dissolved and filtered before submission to avoid interference from large particulates. If special treatment is required for the sample, please notify us in advance.

 

4. Sample Relevant Information: Please provide basic information about the sample, including the sample name, expected molecular weight range, solvent information, and any other special requirements that may affect the analysis.

 

Case Study

1. Gel Permeation Chromatography in Determining Molecular Weights of Lignins: Critical Aspects Revisited for Improved Utility in the Development of Novel Materials

 



Lange, H. et al. ACS SUSTAIN CHEM ENG. 2016.

 

2. Molecular Weight Determination of Low Molecular Weight Hyaluronic Acid and Chondroitin Sulfate by Gel Permeation Chromatography

 



Tian, Z. et al. Carbohydr Polym. 2023. 

 

FAQ

1. How to address sample solubility issues?

To address sample solubility issues, first select a solvent that is compatible with the target molecule, typically using deionized water or buffer solutions. Optimizing solvent conditions, such as temperature and stirring speed, can help improve solubility. If the sample is difficult to dissolve, consider using ultrasonic treatment or solvent mixtures. If necessary, use low-concentration solutions to avoid interference from high-concentration solvents.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Molecular Weight Determination 

5. Mass Spectrometry Image

6. Raw Data