Glycoprotein Modifications Analysis Service

Glycoprotein modifications analysis services use advanced mass spectrometry techniques to accurately analyze the glycosylation modifications on glycoproteins, revealing their types, sites, and changes under different biological conditions. Glycosylation, as an important regulatory mechanism for protein function, directly affects the structure, stability, and biological functions of glycoproteins, especially in processes such as cell recognition, signal transduction, and immune response. Glycoprotein modification analysis not only helps reveal the impact of glycosylation on glycoprotein function but also provides valuable insights for disease mechanism research. Glycosylation abnormalities are often associated with various diseases, such as cancer and diabetes, and analyzing glycosylation changes aids in early diagnosis and targeted therapy. Furthermore, glycoprotein modification analysis plays a key role in drug development, particularly in the development of biopharmaceuticals, where optimizing glycosylation can improve drug efficacy and stability. By deeply analyzing glycoprotein modifications, researchers can better understand the role of glycosylation in cell communication, immune responses, and pathological states, thus advancing the integration of basic research and clinical applications, providing a solid theoretical foundation for discovering novel disease biomarkers and drug development.

 

MtoZ Biolabs' glycoprotein modifications analysis service focuses on studying the glycosylation modifications of glycoproteins and their impact on protein function. Using advanced mass spectrometry technology and efficient separation systems, we provide comprehensive analysis of glycan structures, types, and their variations under different physiological or pathological conditions. This service provides strong support for understanding the critical role of glycosylation in biological processes such as cell recognition, signal transduction, and immune response, with significant applications in the study of cancer, immune, and metabolic diseases. With precise experimental design and high-throughput analysis, MtoZ Biolabs' professional team ensures reliable data, helping to reveal the complex regulation of glycoprotein modifications in biological functions and advancing the development of precision medicine and new drug discovery.

 

Analysis Workflow

 



 

Service Advantages

1. Advanced Technological Advantages: MtoZ Biolabs utilizes cutting-edge mass spectrometry technologies combined with high resolution and accurate mass analysis, enabling clear identification of glycoproteins and their modifications in complex samples, ensuring reliability and accuracy of the data.

 

2. Optimized Analytical Workflow: Our analytical workflow has been streamlined to minimize loss and interference during sample processing, enhancing overall efficiency and allowing clients to quickly obtain high-quality analysis results.

 

3. Precise Differentiation of Glycosylation Types: Utilizing specially developed analytical software, we can accurately identify different types of glycosylation modifications, including N-linked and O-linked glycans, helping researchers gain deeper insights into the biological functions and mechanisms of glycoproteins.

 

4. Expert Team Support: MtoZ Biolabs boasts a highly experienced research team dedicated to providing customized services. From experimental design to data interpretation, we offer comprehensive support to ensure that clients' specific needs are met.

 

Sample Submission Suggestions

 1. Sample Type: Acceptable samples include cell lysates, tissue samples, and purified glycoprotein samples. Samples should be in protein solution form, ensuring the protein concentration is suitable for interaction analysis.

 

2. Buffer Requirements: Samples should be dissolved in buffers appropriate for mass spectrometry analysis, avoiding buffers containing high concentrations of salts, detergents, or protease inhibitors. Common buffers include PBS, Tris-HCl, etc.

 

3. Sample Purity: Samples provided should be of high purity, avoiding contamination with excessive impurities or non-target proteins to ensure the accuracy of the analysis.

 

4. Shipping Requirements: Samples should be shipped according to standard protein sample transport guidelines with cold chain shipping, avoiding exposure to high temperatures or repeated freeze-thaw cycles to maintain sample quality.

 

5. Special Requirements: If the sample contains special glycosylation modifications or complex glycoprotein complexes, please inform us in advance so that we can optimize the analysis plan accordingly.

 

If you need more detailed information or specific analytical methods, feel free to ask!

 

Sample Results

1. Site-Specific N‐Linked Glycosylation Analysis of Human Carcinoembryonic Antigen by Sheathless Capillary Electrophoresis− Tandem Mass Spectrometry

 



Pont, L. et al. J Proteome Res. 2021.

 

2. O- and N-glycosylation analysis of cell lines by ultrahigh resolution MALDI-FTICR-MS

 



Gerda, C.M, Vreeker. et al .INT J MASS SPECTROM. 2020.

 

Applications

1. Pathophysiology Research

 



Ren, W. et al. Front Mol Biosci. 2022.

 

FAQ

1. In analyzing glycoproteins from various biological sources or species, what criteria should be employed to select optimal enzyme digestion conditions and analytical methods that ensure the acquisition of high-quality mass spectrometry (MS) data? 

Based on the specific characteristics of the target glycoprotein, suitable enzymes (e.g., trypsin or lysozyme) should be selected, with an emphasis on optimizing digestion time and temperature. It is advisable to employ liquid chromatography (LC) coupled with mass spectrometry (MS) techniques to enhance separation and sensitivity, thereby enabling the detection of low-abundance glycoproteins.

 

2. In interpreting mass spectrometry data, how can researchers accurately distinguish between isomers of glycosylation sites and different glycan signals, thus avoiding misinterpretations stemming from homologous or overlapping signals? 

Specialized mass spectrometry data analysis software, such as MaxQuant or PEAKS, should be employed alongside database searches and deduplication strategies to precisely identify glycosylation sites and glycan types. A comparative analysis of spectra obtained under varying experimental conditions, in conjunction with multiple analytical approaches (e.g., MS/MS, HRMS), can serve to mitigate the potential for errors in interpretation.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Glycoprotein Modifications Analysis

5. Mass Spectrometry Image

6. Raw Data 

 

If you are interested in our Glycoprotein modifications analysis services, please feel free to contact us, we will serve you wholeheartedly.