Glycoprotein Structure Analysis Service

Protein glycosylation, a key post-translational modification, plays an essential role in modulating protein functions. Despite its significance, the complexity of glycosylation continues to challenge its thorough characterization in proteomics. This modification is crucial for protein folding, modulating immune responses, and regulating interactions within biological systems. Notably, the variability in glycan chain lengths, their structural components, and the specificity at sialylation sites are of particular interest due to their dynamic nature.

 



Bagdonaite, I. et al. Nat Rev Methods Primers. 2022.

Figure 1. Common Protein Glycosylation Classes and Glycans

 

Services at MtoZ Biolabs

MtoZ Biolabs leverages cutting-edge mass spectrometry technologies to offer comprehensive glycoprotein structural analysis services, encompassing everything from sample preparation to data analysis:

1. Sample Preparation: We utilize optimized extraction methods tailored to each sample type, such as blood, tissues, and cells, to preserve protein integrity.

2. Protein Digestion: We employ enzymes like trypsin to digest proteins into peptides, optimizing them for mass spectrometric analysis.

3. Mass Spectrometry Analysis: Using techniques like MALDI-MS, LC-MS, and NSI-MS/MS, we precisely identify glycosylation sites and their structures.

4. Data Analysis and Interpretation: Our data is interpreted using sophisticated bioinformatics tools, ensuring that our findings are both scientifically robust and accurate.

 

Applications

1. Protein engineering for pharmaceutical development.

2. Identification and validation of biological markers.

3. Investigation of protein glycosylation pathways in understanding disease mechanisms.

 

Sample Submission Requirements

Our services accommodate a diverse array of sample types including 2D gel spots, SDS-PAGE bands, serum, plasma, saliva, cells, microorganisms, and plants.

 

Service Advantages

1. Expertise: Our scientifically proficient team offers detailed glycosylation analyses, empowering your research.

2. Tailored Services: We design specific analytical plans to meet the diverse needs of both standard and complex research projects.

3. Efficiency and Precision: We are committed to delivering comprehensive reports swiftly to facilitate your research's progress.

 

As your trusted partner in glycoprotein analysis, MtoZ Biolabs ensures that all services adhere strictly to industry standards, offering robust support for your scientific inquiries. We invite you to contact us to explore more about our services or to discuss your specific research needs.