Glycosylation Site and Glycan Analysis Service

Glycosylation stands out as one of the key post-translational modifications of proteins, crucial for cellular functions and regulatory mechanisms that influence organismal growth and development. Research indicates that over half of mammalian proteins are glycoproteins, pervasive across diverse cells and tissues. Glycans exhibit considerable structural diversity, including varied branching, linkages, and configurations, imparting a broad range of biological functions vital for health, development, and survival. In the context of disease, alterations in glycosylation patterns are often closely linked to pathological conditions, offering avenues for early diagnosis and therapeutic interventions.

 

Analysis Workflow

At MtoZ Biolabs, we leverage sophisticated liquid chromatography-tandem mass spectrometry (LC-MS/MS) techniques for precise analysis of glycosylation sites and their glycan profiles. Our expert team employs targeted enzymatic methods to ensure comprehensive coverage of the protein sequence, enabling accurate identification and analysis of glycosylation sites. We further enhance peptide fragment resolution using cutting-edge fragmentation technologies such as ETD, HCD, and CID, ensuring detailed and accurate analyses.

 



Gashash, E. A. et al. J Proteome Res. 2017.

Figure 1. Glycosylation Sites and Glycoform Analysis Process

 

Service Advantages

1. Label-Free Site Detection

Our high-efficiency LC-MS/MS technology identifies protein glycosylation sites without the need for prior labeling, maintaining test authenticity and accuracy.

 

2. Detailed Glycan Profiling

We provide comprehensive details for each glycosylation site along with insights into glycan distribution and relative abundance, facilitating deep functional and pathological studies.

 

3. Integrated Qualitative and Quantitative Analyses

By merging qualitative and quantitative approaches, we meticulously investigate both N-linked and O-linked glycosylation, offering extensive data support.

 

4. State-of-the-Art Precision

Utilizing advanced fragmentation methods such as ETD, HCD, and CID enhances sensitivity and precision, capturing even minor glycosylation events.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

1. Biopharmaceutical Development and Quality Control: Our glycosylation analysis services are essential for developing antibody and recombinant protein therapeutics, ensuring precise glycosylation characterization crucial for in-process quality control and guaranteeing the efficacy and safety of the end products.

 

2. Glycan Structural Elucidation: We accurately determine glycosylation sites within glycoproteins—both N-glycosylation and O-glycosylation—and their respective glycan forms, which is critical for elucidating protein functions and their biological mechanisms.

 

3. Disease-Related Studies: Our analyses of glycosylation pattern changes reveal biomarkers associated with various diseases, providing a scientific basis for early diagnostic and therapeutic strategies.

 

4. Bioengineering Enhancement: Researchers utilize our glycosylation insights to optimize bioengineering processes and improve protein therapeutic designs, enhancing efficacy and minimizing adverse reactions.

 

As a global leader in proteomics analysis services, MtoZ Biolabs is committed to delivering the most accurate results and the quickest service response. Our team of professionals, with their rich industry experience and expertise, is equipped to perform high-quality analyses on complex samples. For more details about our services or any technical inquiries, please feel free to contact us; we are eager to assist you.