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    Hesperetin Analysis Service

      Hesperetin is a chemical substance mainly derived from young citrus fruits in the Rutaceae family. Hesperetin is a member of the dihydroflavone group, usually a light yellow needle-shaped crystal or powder. Hesperetin is insoluble in water, soluble in methanol, ethanol, acetone and other organic solvents. Hesperetin has antioxidant, anti-inflammatory, lipid-lowering, anti-tumor and cardiovascular protective effects, and can be used in health care drugs and health products, some cosmetic additives and food. In addition, hesperetin as a pharmaceutical raw material, related drugs can be used for strengthening the stomach, expectorant, antitussive, dispelling wind, diuretic, anti-retrograde and stomach pain.

       

      Technical Principles

      We use high performance liquid chromatography-mass spectrometry (HPLC-MS) technology for hesperetin analysis. HPLC is a liquid chromatography analysis technology, which separates the sample by high performance liquid chromatography, and then detects it, so as to achieve qualitative and quantitative analysis of various components in the sample.

       

      Analysis Workflow

      First, we preprocess the sample and use our newly developed sample preprocessing method. This method is safe, environmentally friendly, fast, and effective, with an average recovery rate of up to 99%. Then, we analyze the sample through HPLC, including the creation of standard curves, the preprocessing of raw data, the absolute quantification of hesperetin, the identification and screening of metabolites, etc. Finally, we perform data analysis, provide purity analysis reports, HPLC raw data files and HPLC data quality checks, metabolite quantification data, etc., to obtain accurate and comprehensive analysis results.

       

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      Figure 1. The Workflow of HPLC-MS Analysis of Hesperetin
       

      Service Advantages

      • Excellent Hesperetin Qualitative and Quantitative Capabilities: We have an automated analysis system that can efficiently and accurately complete qualitative and quantitative analysis to ensure scientifically reliable results.
      • Personalized experimental plan: We can provide one-on-one customized services, assign a professional project manager to analyze the entire project, report progress directly and quickly to customers, and flexibly adjust the project plan according to customer opinions.
      • Full-cycle Quality Control System
      • Professional Data Analysis Skills

       

      Applications

      1. Conducting quantitative analysis of hesperetin content

       

      2. Conducting qualitative analysis of the main types of hesperetin

       

      3. Ensuring product safety and quality

       

      4. Providing a basis for product improvement

       

      Sample Submission Requirements

      1. Fresh Tissue Paper >1 g. If possible, the sample should be immediately frozen in liquid nitrogen after collection, and then transferred to -80°C for storage.

      2. Product Samples.

      3. At least 3 biological replicates.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant HPLC and MS Parameters

      3. Detailed Information on Hesperetin Analysis

      4. HPLC and MS Images

      5. Raw Data

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