Histone 2-Hydroxyisobutyrylation Analysis
- Animal tissue ≥ 2 g
- Plant tissue ≥ 0.4 g
- Serum / Plasma ≥ 2 mL (plasma should be collected using EDTA as an anticoagulant)
- Urine ≥ 10 mL
- Cell ≥ 1 x 10⁸ cells
- Yeast / Microbial samples ≥ 0.4 g
- Protein samples: 2-5 mg (Standard tissue or cell lysis buffers can be used during protein extraction)
Histone post-translational modifications (PTMs) play a critical role in the regulation of gene expression and cellular functions. The N-terminal lysine (Lysine, K) residues are primary targets for various modifications, including acetylation, methylation, and ubiquitination. Histone 2-hydroxyisobutyrylation (2-HIB) is an important post-translational modification characterized by the addition of a hydroxyisobutyryl group to the N-terminal lysine residues. This modification plays a crucial role in regulating chromatin structure and function, influencing gene expression, DNA repair, and cellular signaling pathways.
Research on 2-hydroxyisobutyrylation is still in its early stages, but evidence suggests that this modification plays a key role in many important biological processes, including the cell cycle, cell proliferation, and tumorigenesis. Current studies are focused on revealing its dynamic changes and interactions with other PTMs (such as acetylation and phosphorylation) to gain a deeper understanding of the complex networks regulating cellular functions.
Recent advancements in mass spectrometry and proteomics have significantly advanced research on histone 2-hydroxyisobutyrylation. Researchers are increasingly focusing on the biological significance of this modification and its potential implications for various diseases. However, the complexity of histone post-translational modifications (PTMs) presents substantial challenges to analytical techniques. One major difficulty lies in the isolation and purification of pure histone components from the cell nucleus, which is essential for accurately characterizing histone modifications. Additionally, the analysis of post-translational modifications on N-terminal lysine residues faces considerable challenges due to the presence of multiple modifications on individual lysine residues.
Services at MtoZ Biolabs
MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to provide a comprehensive solution for histone 2-hydroxyisobutyrylation analysis. Simply provide your experimental objectives and send us your samples, and we will offer you a one-stop service from sample preparation and MS analysis to data analysis, delivering detailed identification and quantitative analysis of histone 2-hydroxyisobutyrylation modifications and their modification sites.
Compared to traditional proteomics analysis, the key to histone modification analysis lies in the isolation and purification of histone components, as well as mitigating the impact of N-terminal lysine on the analysis of histone post-translational modifications. Since histones are located in the cell nucleus, MtoZ Biolabs has developed a specialized platform for histone extraction and purification, drawing on existing literature and experimental experience. Cells are homogenized and subjected to low-speed centrifugation to obtain the nuclei. The nuclei are then further disrupted to release histones and DNA. High-salt buffer is used to remove impurities, and High-Performance Liquid Chromatography (HPLC) is employed to separate different histone components, achieving optimal purity for subsequent mass spectrometry analyses. Additionally, to eliminate the impact of N-terminal lysine, we utilize 2-3 different enzymes to enhance peptide coverage in mass spectrometry analysis during the digestion process. This multi-enzymatic digestion increases the coverage of histone peptides, preventing the loss of post-translational modification information due to unsuitable peptide lengths or low ionization efficiency.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform
Mtoz Biolabs established an advanced histone 2-hydroxyisobutyrylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all histone 2-hydroxyisobutyrylation analysis data providing clients with acomprehensive data report.
4. Optimized Extraction
An optimized histone extraction and purification protocols are employed to minimize contamination and ensure high purity.
Sample Submission Requirements
1. Sample Volume
2. Sample Preservation
Samples should be shipped with dry ice. Avoid repeated cycles of freezing and thawing
Note: We will conduct testing on the provided samples. The formal experiment will commence only after the samples pass the quality check.
Deliverables
1. Comprehensive Experimental Details (Materials, Instruments, and Methods, etc.)
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Histone 2-Hydroxyisobutyrylation Analysis
4. Raw Data
5. Mass Spectrometry Image
6. Custom Analysis Report
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our histone 2-hydroxyisobutyrylation analysis services, please feel free to contact us. We are dedicated to providing you with the best service.
How to order?
