Histone Post-Translational Modification Analysis Service

Histones are highly conserved proteins, and histone subtypes have highly similar amino acid sequence. The N-terminal ends of histones can be modified by several types of PTMs, including acetylation, methylation, phosphorylation and so on, which affect the DNA transcription, replication, and DNA condensation, etc. Therefore, analysis on histone N-terminal modifications can provide valuable information for epigenetic studies. MtoZ Biolabs has optimized our sample preparation protocols to obtain highly purified histones with the least effect to the modification. We also use 2-3 enzymes for protein digestion to ensure full coverage analysis of histone sequence, eliminating any missing information of short peptides rendered by using only one enzyme.

MtoZ Biolabs has developed a specialized platform, equipped with Q Exactive HF (Thermo Fisher), Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometers, equipped with Nano-LC for histone modification analysis service. To better solve your research problems, we offer an all-inclusive service, including sample preparation, protein purification, digestion, peptide separation, LC-MS/MS analysis, histone modification analysis and bioinformatics analysis. All you need to do is to tell us your project objective, and send us your cell samples, and we will complete all the following experiments.

 

Analysis Workflow

 


 

Sample Submission Requirements

 


*Note: We will perform testing experiment before we start official experiments. To ensure the most cost-effective and accurate analysis is provided, only qualified samples will proceed to the official analysis.

 

Deliverables

1. Experiment Procedures
2. Parameters of Liquid Chromatography and Mass Spectrometer
3. MS Raw Data Files
4. Peptide Identifications and Intensities
5. Protein Identifications and PTMs Mapping
6. Bioinformatics Analysis

 

Related Services

PTM Analysis

Phospho Proteomics
Acetyl Proteomics
Ubiquitin Proteomics
Glyco Proteomics
Disulfide Bond
Histone Modifications



Protein Analysis

Protein Identification
Protein Mass Measurement
PTMs Identification



Protein Sequencing

Protein De Novo Seq
N-Terminal Sequencing
C-Terminal Sequencing
Edman Degradation
Protein Full-Length Sequencing

• • Histone Lysine Methacrylation Analysis

  Histone lysine methacryloylation (Kcr) is a new type of post-translational modification (PTM), which is characterized by the covalent binding of a methacryloyl group (-CH=CH-CH3) to a lysine residue. The analysis of histone lysine methacryloylation is of great significance in gene regulation, disease mechanisms, epigenetics, and drug development. It not only provides a new perspective for understanding the basic processes of life, but also provides important targets and biomarkers for the early diagnosis an

• • Histone Methionylation Analysis

  Histone methionylation refers to the post-translational modification of methionine residues on histones. This modification typically occurs at the N-terminus of histones or on specific lysine residues, influencing gene expression and chromatin structure. The analysis of histone methionylation is of great significance in fundamental biology and medical research, helping us understand gene expression regulation, cell fate determination, and disease mechanisms. By conducting in-depth studies in this area, scie

• • Histone Guanidination Analysis

  Histone guanidinylation is a relatively uncommon post-translational modification (PTM) involving the covalent addition of a guanidino group to specific amino acid residues in histones, primarily arginine residues. While research into histone guanidinylation is still in its infancy, its potential involvement in regulating gene expression, chromatin remodeling, and DNA repair has positioned it as a growing area of interest in epigenetic studies. As more is uncovered about the mechanisms underlying guanidinyla

• • Histone Asymmetric Dimethylation Analysis

  Histone asymmetric dimethylation is a post-translational modification (PTM) that involves the addition of two asymmetric methyl groups to arginine residues on histones, where two methyl groups are added to one nitrogen atom of arginine, while the other nitrogen atom remains unmodified. Histone asymmetric dimethylation plays a key role in gene expression, chromatin regulation, DNA repair, and cell fate determination. By conducting in-depth studies on this modification, scientists can uncover its mechanisms i

• • Histone Crotonylation Analysis

  Histone crotonylation is a critical post-translational modification that involves the addition of a crotonyl group to lysine residues on histone proteins. This modification has significant implications for gene expression, chromatin dynamics, and cellular metabolism. Crotonylation is primarily catalyzed by histone crotonyltransferases, which utilize crotonyl-CoA as a substrate. Crotonyl-CoA is produced from crotonate, a short-chain fatty acid, through enzymatic pathways involving acyl-CoA synthetase.

• • Histone Symmetric Dimethylation Analysis

  Histone symmetric dimethylation is a crucial post-translational modification that significantly influences gene expression, chromatin structure, and cellular function. This modification involves the addition of two methyl groups to specific lysine or arginine residues on histones, resulting in a symmetric configuration. The process of symmetric dimethylation is catalyzed by histone methyltransferases (HMTs), which utilize S-adenosylmethionine (AdoMet) as a methyl donor. 

• • Histone Monomethylation Analysis

  Histone monomethylation is an important post-translational modification that plays a critical role in the regulation of gene expression, chromatin structure, and cellular processes. This modification involves the addition of a single methyl group to specific lysine or arginine residues on histone proteins, primarily within the N-terminal tails of histones H3 and H4. Histone monomethylation is catalyzed by histone methyltransferases (HMTs), which transfer methyl groups from S-adenosylmethionine (AdoMet) to t

• • Histone Dimethylation Analysis

  Histone dimethylation is a crucial post-translational modification that involves the addition of two methyl groups to specific lysine or arginine residues on histone proteins. This modification plays a pivotal role in regulating gene expression, chromatin structure, and overall cellular function. Histone dimethylation can either activate or repress gene expression, depending on the specific residue being modified and the context of other concurrent modifications.

• • Histone Lactylation Analysis

  Lactate is a well-known and ubiquitous molecule, recognized primarily as a byproduct of the glycolytic pathway. In the complex landscape of glucose oxidation metabolism, pyruvate plays a pivotal role, being enzymatically converted to L-lactate by lactate dehydrogenase (LDH). This conversion is a common energy-generating process that occurs extensively in mammalian cells.

• • Histone Acetylation Analysis

  Histone acetylation is a pivotal post-translational modification that significantly influences chromatin structure and gene expression. This modification involves the addition of an acetyl group (−COCH₃) to the lysine residues on histone proteins. This modification occurs when histone acetyltransferases (HATs) utilize acetyl-CoA as a cofactor to acetylate lysine residues on histone proteins.