Histone Monomethylation Analysis
- Animal tissue ≥ 2 g
- Plant tissue ≥ 0.4 g
- Serum / Plasma ≥ 2 mL (plasma should be collected using EDTA as an anticoagulant)
- Urine ≥ 10 mL
- Cell ≥ 1 x 10⁸ cells
- Yeast / Microbial samples ≥ 0.4 g
- Protein samples: 2-5 mg (Standard tissue or cell lysis buffers can be used during protein extraction)
Histone monomethylation is an important post-translational modification that plays a critical role in the regulation of gene expression, chromatin structure, and cellular processes. This modification involves the addition of a single methyl group to specific lysine or arginine residues on histone proteins, primarily within the N-terminal tails of histones H3 and H4. Histone monomethylation is catalyzed by histone methyltransferases (HMTs), which transfer methyl groups from S-adenosylmethionine (AdoMet) to the target residues.
Monomethylation of specific residues can serve as a signal for the recruitment of various transcription factors and regulatory proteins that influence gene expression. For instance, H3K4me1 is associated with enhancers and is crucial for transcriptional activation, while H3K9me1 can play a role in the regulation of gene silencing and chromatin compaction. Histone monomethylation is involved in numerous biological processes, including development, differentiation, and the cellular response to environmental changes. The specific methylation patterns and levels can dictate whether genes are activated or silenced, thereby contributing to cellular identity and function.
Figure 1. Models on the Roles of H3K4me1 in Gene Regulation
Additionally, dysregulation of histone monomethylation has been implicated in various diseases, including cancer and developmental disorders. Altered expression or activity of HMTs and the presence of abnormal monomethylation patterns can lead to aberrant gene expression profiles that drive tumorigenesis. Understanding the precise roles and mechanisms of histone monomethylation can provide valuable insights into potential therapeutic strategies targeting these modifications.
Services at MtoZ Biolabs
MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to provide a comprehensive solution for histone monomethylation analysis. Simply provide your experimental objectives and send us your samples, and we will offer you a one-stop service from sample preparation and MS analysis to data analysis, delivering detailed identification and quantitative analysis of histone monomethylation modifications and their modification sites.
Compared to traditional proteomics analysis, the key to histone modification analysis lies in the isolation and purification of histone components, as well as mitigating the impact of N-terminal lysine on the analysis of histone post-translational modifications. Since histones are located in the cell nucleus, MtoZ Biolabs has developed a specialized platform for histone extraction and purification, drawing on existing literature and experimental experience. Cells are homogenized and subjected to low-speed centrifugation to obtain the nuclei. The nuclei are then further disrupted to release histones and DNA. High-salt buffer is used to remove impurities, and High-Performance Liquid Chromatography (HPLC) is employed to separate different histone components, achieving optimal purity for subsequent mass spectrometry analyses. Additionally, to eliminate the impact of N-terminal lysine, we utilize 2-3 different enzymes to enhance peptide coverage in mass spectrometry analysis during the digestion process. This multi-enzymatic digestion increases the coverage of histone peptides, preventing the loss of post-translational modification information due to unsuitable peptide lengths or low ionization efficiency.
Analysis Workflow
Service Advantages
1. Advance Analysis Platform
Mtoz Biolabs established an advanced histone monomethylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge
Our pricing is transparent, no hidden fees or additional costs.
3. High-Data-Quality
Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all histone monomethylation analysis data providing clients with acomprehensive data report.
4. Optimized Extraction
An optimized histone extraction and purification protocols are employed to minimize contamination and ensure high purity.
Sample Submission Requirements
1. Sample Volume
2. Sample Preservation
Samples should be shipped with dry ice. Avoid repeated cycles of freezing and thawing
Note: We will conduct testing on the provided samples. The formal experiment will commence only after the samples pass the quality check.
Deliverables
1. Comprehensive Experimental Details (Materials, Instruments, and Methods, etc.)
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on Histone Monomethylation Analysis
4. Raw Data
5. Mass Spectrometry Image
6. Custom Analysis Report
MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our histone monomethylation analysis services, please feel free to contact us. We are dedicated to providing you with the best service.
How to order?
