Small Molecule Drug Targets Identification Service | ABPP

Small molecule drugs play a very important role in the treatment of diseases, and their mechanism of action is to exert their function by interacting with specific target molecules in cells. These target molecules are mainly proteins, including enzymes, ion channel proteins, and other types. Therefore, the identification of the target protein of small molecule drug action is crucial for understanding the mechanism of action and application of small molecule drugs, and can also further discover the off-target targets, side effects, and biological functions involved in the target protein of small molecule drugs.

 

Traditional methods for identifying drug targets use chemical modification-based approaches and label-free target identification methods. Chemical modification methods include affinity chromatography, active site-directed probe techniques, and protein microarray technologies among others. There are certain limitations to chemical modification-based methods, as they all require labeling or derivation of small molecule drugs or protein libraries, while small molecule drugs may lack sites for covalent cross-linking, or chemical modification may affect the binding between drugs and target proteins. Label-free target identification methods do not involve any chemical modification of small molecule drugs and can complement methods based on chemical modification. Many studies have applied label-free methods, including cellular thermal shift assay (CETSA), thermal proteome profiling (TPP), pulse proteolysis (PP), stability of proteins from rates of oxidation (SPROX), drug affinity responsive target stability (DARTS), limited proteolysis coupled to mass spectrometry (LiP-MS), and solvent-induced protein precipitation (SIP), which can identify multiple targets of small molecule drugs simultaneously.

 

MtoZ Biolabs boasts a comprehensive analytical platform for small molecule drug targets, featuring an array of high-resolution mass spectrometers and auxiliary equipment like high-performance liquid chromatography. This platform is equipped with extensive data storage, retrieval, and analysis capabilities. The services offered by the platform include extraction of proteins from various sources, incubation of drugs with purified protein/cell/tissue samples, sample pre-treatment for mass spectrometry, sample fractionation by high-performance liquid chromatography, and mass spectrometry data collection, enabling identification and quantitative research of small molecule drug targets. The team can explore the best experimental conditions through multiple gradient experiments, collect data through mass spectrometry, and analyze detailed information on drug targets through bioinformatics analysis. MtoZ Biolabs can provide services such as protein expression, protein extraction, drug treatment, sample preparation, mass spectrometry data collection, and experimental data analysis, offering a one-stop solution for the identification and verification of small molecule drug targets.