IR Spectral Analysis Principles: Revealing Information from Spectra to Structure

Infrared spectroscopy analysis is a commonly used analytical technique which obtains information about the structure and chemical composition of samples by measuring their absorption and scattering in the infrared light band.

 

Infrared spectroscopy refers to the absorption and scattering phenomena of substances in the infrared light band (wavelength range of 0.78-1000 micrometers). The infrared spectrometer obtains the infrared spectrum by irradiating the sample with infrared light and measuring the sample's absorption of light at different wavelengths.

 

Characteristics of Infrared Spectroscopy

Infrared spectroscopy has the following characteristics:

1. Infrared spectroscopy can provide structural information of substances. Different chemical bonds and functional groups show specific absorption peaks in the infrared spectrum. By analyzing the position and intensity of the absorption peaks, the chemical bonds and functional groups present in the sample can be inferred.

2. Infrared spectroscopy has low requirements for samples. Infrared spectroscopy can analyze solid, liquid, and gas samples, and the sample preparation is relatively simple, without the need for complex preprocessing steps.

3. Infrared spectroscopy analysis is fast. Infrared spectrometers can obtain spectral information of samples in a short time, suitable for high-throughput analysis.

 

Relationship Between Infrared Spectroscopy and Structure

The absorption peaks in the infrared spectrum are related to the chemical bonds and functional groups in the sample. Different chemical bonds and functional groups show specific absorption peaks in the infrared spectrum, and the position and intensity of these peaks can provide structural information.

 

For example, the vibration of the C-H bond usually shows a strong absorption peak in the infrared spectrum, and its position and shape can be used to determine the structure of hydrocarbons. Similarly, the vibration of the C=O bond usually shows a strong absorption peak in the infrared spectrum, and its position and intensity can be used to determine the presence of ketones, aldehydes, and other compounds.

 

Applications

Infrared spectroscopy analysis has a wide range of applications in the field of biopharmaceuticals. Here are some common application fields:

 

1. Drug Development

Infrared spectroscopy can be used to analyze the structure and purity of drugs, helping developers determine the quality and potency of drugs.

 

2. Protein Structure Research

Infrared spectroscopy can be used to study the secondary and tertiary structure of proteins, helping scientists understand the function and interaction of proteins.

 

3. Drug Interaction Research

Infrared spectroscopy can be used to study the interaction between drugs and their targets, helping scientists design more effective drugs.

 

Infrared spectroscopy analysis is an important analytical technique which obtains information about the structure and chemical composition of samples by measuring their absorption and scattering in the infrared light band. Infrared spectroscopy analysis has a wide range of application prospects in drug development, protein structure research, and drug interaction research. By analyzing the infrared spectrum, we can reveal structural information from the spectrum, providing strong support for scientific research and drug development.