Isobaric Tag for Relative and Absolute Quantitation
Isobaric Tag for Relative and Absolute Quantitation is an advanced mass spectrometry technique used in proteomics research, enabling the relative or absolute quantification of proteins in various samples through isotope labeling. Isobaric tag for relative and absolute quantitation allows researchers to measure protein expression changes under different experimental conditions, facilitating the exploration of biological processes such as cell signaling, disease mechanisms, and drug responses. Additionally, isobaric tag for relative and absolute quantitation can identify and quantify proteins within organisms, providing a comprehensive and systematic analysis of the proteome. These features make isobaric tag for relative and absolute quantitation an invaluable tool in biomedical research, clinical diagnostics, and drug development. In basic research, isobaric tag for relative and absolute quantitation is employed to uncover the molecular mechanisms of diseases, including cancer and neurodegenerative disorders. It also plays a crucial role in biomarker discovery by analyzing samples from diseased patients and healthy controls to identify potential diagnostic markers or therapeutic targets. In drug development, isobaric tag for relative and absolute quantitation technology is used to assess the effects of drugs on protein expression, screen for drug targets, optimize drug structures, and enhance drug efficacy and safety. Furthermore, isobaric tag for relative and absolute quantitation is instrumental in drug metabolism research, aiding in the understanding of drug metabolic pathways and kinetics within the body.
Analysis Workflow of Isobaric Tag for Relative and Absolute Quantitation
The isobaric tag for relative and absolute quantitation workflow involves several key steps, including protein extraction, enzymatic digestion, labeling, separation, and mass spectrometric analysis. Initially, proteins are extracted from biological samples and digested into peptides. Isobaric tag for relative and absolute quantitation reagents, which consist of isotopically labeled chemical groups, are then used to label these peptides by reacting with their N-termini or lysine side chains. This labeling allows multiple samples to be mixed for analysis. Post-labeling, the samples are separated using High-Performance Liquid Chromatography (HPLC) and analyzed by mass spectrometry. The mass spectrometer quantifies each peptide by detecting the mass-to-charge ratio (m/z) of peptide fragments. Mass spectrometry data processing software is then used to obtain quantitative information on proteins in the samples. The entire process requires precise experimental execution to ensure accurate and reproducible quantitative results.
Advantages and Limitations of Isobaric Tag for Relative and Absolute Quantitation
Isobaric tag for relative and absolute quantitation technology offers high-throughput and sensitive quantitative protein analysis. Unlike traditional methods, isobaric tag for relative and absolute quantitation can simultaneously analyze multiple samples, significantly enhancing experimental efficiency. Its high sensitivity enables the detection of low-abundance proteins, crucial for examining key proteins in complex biological samples. However, isobaric tag for relative and absolute quantitation has certain limitations. Its labeling reactions demand stringent experimental conditions to avoid incomplete labeling or sample loss. Additionally, the mass spectrometric analysis requires high-performance instruments and experienced operators. The complex data analysis inherent to isobaric tag for relative and absolute quantitation necessitates robust data processing software and computational resources. Despite these challenges, isobaric tag for relative and absolute quantitation remains a vital tool in proteomics research.
MtoZ Biolabs offers professional label-based protein quantification services, including isobaric tag for relative and absolute quantitation, TMT, and SILAC. Our experienced scientific team and advanced technical support ensure high-quality protein quantification analysis. We aim to provide comprehensive technical support for our clients' research, aiding them in achieving breakthroughs in proteomics. We welcome collaboration to explore the limitless possibilities of proteomics.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
How to order?
