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    Isorhamnetin Analysis Service

      Isorhamnetin is a dietary flavonol, naturally found in various plants, including herbs and fruits. It is a member of the flavonoids family, a group of plant-derived polyphenolic compounds known for their potent antioxidant properties. Isorhamnetin has been shown to possess various beneficial effects, including anti-cancer, anti-inflammatory, hypolipidemic, anti-diabetic, and neuroprotective properties. The compound is typically a yellowish crystalline powder, and is soluble in chloroform, acetone, and methanol, but insoluble in water.

       

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      Figure 1. The Structure of Isorhamnetin
       

      Technical Principles

      The Isorhamnetin Analysis Service at MtoZ Biolabs utilizes advanced high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) for the analysis of isorhamnetin. This technique is capable of separating, identifying, and quantifying this compound with high accuracy and sensitivity. The combination of HPLC separation and MS detection allows for the accurate measurement of isorhamnetin, even in complex mixtures and at low concentrations.

       

      Analysis Workflow

      The procedure involves the extraction and purification of isorhamnetin from the sample, followed by analysis using HPLC-MS. The sample is first subjected to a specialized extraction process, designed to efficiently extract isorhamnetin while minimizing the co-extraction of interfering compounds. The extract is then purified to further remove any potential interferences. The purified extract is then analysed by HPLC-MS.

       

      1818493345885401088-WorkflowforIsorhamnetinbyHPLC-MSAnalysis.png

      Figure 2. Workflow for Isorhamnetin by HPLC-MS Analysis

       

      Service Advantages

      MtoZ Biolabs offers a comprehensive Isorhamnetin Analysis Service, delivering accurate and reliable results with a quick turnaround. Our team of experienced scientists and technicians are committed to providing the highest level of service, from initial sample submission to final data analysis and reporting. We also offer a wide array of additional services to complement our Isorhamnetin Analysis Service, such as metabolomics and proteomics analysis, to provide a comprehensive solution for your research needs.

       

      Applications

      Isorhamnetin Analysis Service is applicable for various types of samples, including biological tissues, dietary supplements, and food products. The reliable quantification of isorhamnetin is crucial for studies investigating the bioavailability, metabolism, and pharmacokinetics of this important flavonol.

       

      Sample Submission Requirements

      1. Fresh Tissues >1 g. If possible, samples should be frozen in liquid nitrogen immediately after collection, and then transferred to -80°C for storage.

       

      2. Dietary Supplement and Food Product Samples.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Isorhamnetin Analysis

      4. Mass Spectrometry Images

      5. Raw Data

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