iTRAQ and TMT Label-Based Quantitative Proteomics Utilize Mass Spectrometry Information for Quantitative Analysis
In proteomics research, iTRAQ (Isobaric Tag for Relative and Absolute Quantitation) and TMT (Tandem Mass Tags) are widely used for quantitative analysis. These methods involve labeling proteins or peptides with tags of identical mass, allowing quantification through the intensity of reporter ions during mass spectrometry.
Reporter Ion Intensity
The intensity of reporter ions is crucial for iTRAQ and TMT quantification. During MS/MS analysis, these ions are released upon fragmentation. Due to the distinct chemical compositions of each tag, the mass-to-charge ratios of the corresponding reporter ions differ. Consequently, the intensities of the reporter ions directly reflect the abundance of the original peptides.
Variability in Peptide Ionization Efficiency
While tags of the same mass are expected to exhibit uniform ionization efficiency, in practice, variations in their chemical structure can lead to differences. This affects reporter ion intensity and, subsequently, the accuracy of quantification. Therefore, it is essential to consider ionization efficiency when employing these tags in quantitative analyses.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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