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    Jasmonates (JAs) Analysis Service

      Jasmonates (JAs) are a series of crucial plant hormones primarily involved in mediating plant defense responses. Derived from fatty acids, JA compounds include JA, jasmonoyl-isoleucine conjugate (JA-ILE), methyl jasmonate (MEJA), dihydrojasmonic acid (H2JA), and 12-oxo-phytodienoic acid (12-OPDA). For a deeper understanding of JA signaling and metabolic pathways, precise detection and quantitative analysis of JA and its conjugates are particularly important. Traditionally, GC-MS has been used for analyzing JAs. However, this method poses challenges due to the separation of high boiling point compounds and potential thermal decomposition of molecules during long chromatographic separations at high temperatures, complicating the quantification of small amounts of JAs. In contrast, LC-MS/MS is now widely used for the determination of small metabolites like JA and its derivatives due to its rapidity and accuracy.

       

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      Reyes-Díaz M, et al. Molecules. 2016.

      Figure 1. Methyl Jasmonate Biosynthesis

       

      Services at MtoZ Biolabs

      At MtoZ Biolabs, we utilize cutting-edge LC-MS/MS technology to specifically carry out precise quantitative analysis of JA in various types of samples. Our JA analysis services are both efficient and cost-effective, capable of significantly advancing your research from exploring plant physiological mechanisms to enhancing crop health and yield. With our expertise, you can quickly and accurately assess the activity of JA, providing a scientific basis for crop improvement and disease management.

       

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      Applications

      • Identification of plant stress bio-markers
      • Improvement in crop health and yield
      • Accelerate plant physiology research
      • Uncover new mechanisms in plant stress responses

       

      Service Advantages

      1. Efficient Sample Processing: Optimized pre-treatment steps ensure efficient and accurate recovery of JA and its derivatives from various plant samples.

      2. Broad Detection Capabilities: Capable of quantitatively analyzing JA and its various forms, such as MEJA, H2JA, JA-ILE, and 12-OPDA.

      3. Precision Technology Platform: Utilizing advanced LC-MS/MS technology ensures high sensitivity and accuracy of analysis.

      4. Personalized Data Analysis: Customized data processing and bioinformatics interpretation based on project needs, helping clients deeply understand experimental data.

      5. Cost-Effectiveness: An integrated service process provides a high value-for-money solution, meeting a wide range of scientific and commercial needs.

       

      Sample Submission Requirements

      We can analyze a range of plant materials including but not limited to leaves, stem, flowers, and fruits. If you need to ship samples to us, please follow the requirements for different types of samples:

      • Leaves/Stem: 200mg/sample
      • Flowers/Fruits: 500mg/sample
      • Shipping Condition: Dry Ice

       

      Deliverables

      1. Experimental plan

      2. Instrumentation parameters for LC-MS/MS

      3. Raw data files of LC-MS/MS and a summary of data quality

      4. Bioinformatics analysis report

       

      With our advanced JAs analysis platform, professional bioinformatics software, and experienced technicians and scientists, MtoZ Biolabs offers clients customized JAs analysis services with rapid experimental procedures and easy-to-read reports to accelerate your scientific research.

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