Ketone Bodies Metabolism Analysis Service
- Understanding the role of ketone bodies in various diseases like diabetes, cancer, and neurological disorders.
- Investigating metabolic changes during fasting and exercise.
- Evaluating the effect of ketogenic diets on ketone body metabolism.
- We accept a variety of sample types including serum, plasma, urine, and tissue samples.
- Minimum volume required: 200 µl for biofluid samples, 500 mg for tissue samples.
- Samples should be stored in a -80℃ environment and transported on dry ice.
Ketone bodies are small molecules produced during the metabolism of fatty acids, primarily in the liver. They serve as an alternative energy source during periods of fasting, prolonged exercise, or carbohydrate restriction. The three primary ketone bodies are acetone, acetoacetate, and β-hydroxybutyrate. These compounds are crucial for maintaining energy homeostasis, especially in tissues like the brain and muscles that rely heavily on glucose under normal conditions.
The metabolism of ketone bodies, known as ketogenesis and ketolysis, involves a series of enzymatic reactions. In ketogenesis, fatty acids are broken down into acetyl-CoA, which is then converted into ketone bodies. This process primarily takes place in the mitochondrial matrix of liver cells. Key enzymes involved in ketogenesis include 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS2) and 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL). The produced ketone bodies are released into the bloodstream, where they are transported to other tissues.
Ketolysis occurs in peripheral tissues, where ketone bodies are converted back into acetyl-CoA, entering the tricarboxylic acid (TCA) cycle to produce energy. This process is mediated by enzymes such as succinyl-CoA:3-ketoacid CoA transferase (SCOT) and β-hydroxybutyrate dehydrogenase (BDH). Understanding the regulation of ketone body metabolism is vital for insights into metabolic conditions such as diabetes, obesity, and metabolic syndrome.
Services at MtoZ Biolabs
Our Ketone Bodies Metabolism Analysis Service utilizes liquid chromatography-mass spectrometry (LC-MS) or gas chromatography-mass spectrometry (GC-MS) to identify and quantify ketone bodies such as acetone, acetoacetate, and beta-hydroxybutyrate, providing a comprehensive understanding of metabolic pathways.
Analysis Workflow
Service Advantages
1. Accurate and Reliable Data
Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.
2. Expert Team
A team of highly skilled scientists and technical experts, with extensive experience, ensures the delivery of exceptional results.
3. Cutting-Edge Technology
Utilizes state-of-the-art mass spectrometry technology to offer highly sensitive and accurate measurements, suitable for detecting ketone bodies.
4. Customizable Service
Personalized service to meet various research needs.
Applications
Our Ketone Bodies Metabolism Analysis Service can be applied in various research fields including but not limited to:
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Ketone Bodies Metabolism Analysis
4. Raw Data
5. Custom Analysis Report
With MtoZ Biolabs, you can be confident to receive a comprehensive analysis of ketone bodies metabolism, paving the way towards your groundbreaking research discoveries. Contact us today for further information about our service.
How to order?
