L-Valine Analysis Service

L-Valine is an essential amino acid that must be obtained through diet as the human body cannot synthesize it. It plays a crucial role in protein synthesis, tissue repair, and energy metabolism, particularly in muscle metabolism and immune function. During periods of stress and exercise, the demand for L-valine increases, making it vital to maintain adequate levels for overall health. The metabolic pathway of L-valine involves its conversion to α-ketoisovaleric acid, which then enters the citric acid cycle for energy production. Additionally, L-valine's metabolite, β-hydroxy-β-methylbutyrate (HMB), is an important metabolic regulator that helps reduce protein breakdown and promotes muscle growth and repair. Thus, L-valine metabolism is essential for maintaining muscle mass and overall metabolic health.

 

Changes in L-valine levels can indicate various physiological and pathological states, such as liver diseases, metabolic syndrome, and neurodegenerative diseases. Accurate analysis of L-valine levels and their fluctuations in these conditions aids in understanding its role in health and disease, providing a scientific basis for clinical diagnosis and treatment. Therefore, precise analysis of L-valine is of significant importance in biomedical research and clinical diagnostics.

 



Figure 1. Molecular Structure of L-Valine

 

MtoZ Biolabs utilizes liquid chromatography-tandem mass spectrometry (LC-MS/MS) to conduct detailed quantitative and qualitative analyses of L-valine and its metabolites. This advanced method integrates LC's rapid separation capabilities with the high detection sensitivity of MS/MS, facilitating the precise measurement of L-valine in complex biological matrices.

 

Analysis Workflow

 

 

 

Service Advantages

 



 

Sample Submission Requirements

Sample Types

Serum, plasma, urine, tissue homogenates, and other biological samples.

 

Sample Volume

At least 200 μL of liquid sample or 200 mg of tissue sample.

 

Sample Preservation

Store and transport samples at -80°C to maintain L-valine stability.

 

Applications

Clinical Diagnosis: Detect L-Valine levels in patients with liver diseases, metabolic syndrome, and neurodegenerative diseases to assist in diagnosis and prognosis.

 

Metabolic Research: Investigate the role of L-Valine in protein synthesis, tissue repair, and energy metabolism.

 

Drug Development: Assess the impact of drugs on L-Valine metabolism to aid in developing new therapeutic agents.

 

Nutritional Research: Analyze the metabolic changes of L-Valine under different dietary conditions, providing scientific evidence for nutritional regulation and dietary supplementation.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on L-Valine and their Metabolites

4. Raw Data

5. Custom Analysis Report