Lithocholic Acid Analysis Service

Lithocholic acid (LCA), also known as 3α-hydroxy-5β-cholan-24-oic acid, is a secondary bile acid produced from deoxycholic acid by gut microbiota. LCA is essential in the bile acid cycle, primarily acting as a detergent to solubilize fats for absorption. It also plays a role in lipid metabolism, cholesterol reabsorption, and maintaining bile acid homeostasis. However, the accumulation of LCA can be toxic, and it has been linked to carcinogenesis in both humans and experimental animals.

 

Preliminary in vitro studies have shown that LCA selectively kills neuroblastoma cells while sparing normal neuronal cells, and it is cytotoxic to several other malignant cell types at physiologically relevant concentrations. These findings suggest potential therapeutic applications for LCA, although its toxic risks warrant careful consideration. Therefore, accurate analysis of LCA and its metabolic pathways is crucial for understanding its biological functions and for its use in clinical and research applications.

 

Figure 1. Molecular Structure of Lithocholic Acid

 

MtoZ Biolabs offers Lithocholic Acid Analysis Service using high performance liquid chromatography-mass spectrometry (HPLC-MS). This advanced method combines the separation capabilities of HPLC with the detection power of MS, ensuring high sensitivity and specificity in LCA detection.

 

Analysis Workflow

 



 

Service Advantages

1. High Sensitivity and Precision

HPLC-MS/MS provides highly sensitive and accurate measurements, suitable for detecting low concentrations of LCA.

 

2. Rapid and Efficient

Optimized sample preparation and fast analysis ensure quick and efficient processing.

 

3. Wide Applicability

Suitable for various biological samples, including blood, urine, feces, and tissues.

 

4. Accurate and Reliable Data

Ensures high reproducibility and low error rates, supporting both research and clinical applications, providing reliable and specific results.

 

Sample Submission Requirements

Make sure the sample volume is greater than 0.2 g or 0.2 mL. For detailed sample submission information, please contact our customer service.

 

Applications

1. Liver and Bile Duct Disease Research: Study LCA level changes to understand its role in conditions such as gallstones and cholestasis.

 

2. Cholesterol Metabolism Research: Investigate LCA's involvement in cholesterol metabolism, providing insights for managing hypercholesterolemia.

 

3. Lipid Metabolism Research: Examine LCA's function in lipid digestion and absorption, revealing its potential role in metabolic disorders.

 

4. Cancer Research: Explore LCA's selective cytotoxicity against malignant cells to assess its potential in cancer treatment.

 

5. Toxicity Assessment: Assess the toxic effects of LCA accumulation, particularly in cases of abnormal bile acid metabolism.

 

Deliverables

1. Experimental Procedures

2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Lithocholic Acid

4. Raw Data

5. Custom Analysis Report

 

Our Lithocholic Acid Analysis Service provides high-quality data support, advancing your scientific research and applications. For any inquiries or requirements, please contact us.