Malic Acid Analysis Service

    Malic acid is a dicarboxylic acid produced by all living organisms. It contributes to the sour taste of fruits and is widely used as a food additive. Malic acid exists in two stereoisomeric forms (L- and D-enantiomers), with only the L-isomer occurring naturally. The salts and esters of malic acid are known as malates, which serve as metabolic intermediates in the citric acid cycle. In human metabolism, malic acid plays a critical role by participating in the tricarboxylic acid (TCA) cycle, a key component of energy production. Besides its biological functions, malic acid is used as an acidulant and preservative in the food industry and has significant applications in cosmetics and pharmaceuticals. Given its wide applications and essential biological roles, accurate quantitative and qualitative analysis of malic acid is crucial in food safety, nutritional research, and clinical diagnostics.

     

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    Figure 1. Molecular Structure of Malic Acid

     

    MtoZ Biolabs offers Malic Acid Analysis Service using ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). This advanced method combines the separation capabilities of UPLC with the detection power of MS, ensuring high sensitivity and specificity in malic acid detection.

     

    Analysis Workflow

     

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    Service Advantages

    1. High Sensitivity

    Detects very low concentrations of malic acid accurately.

     

    2. High Specificity

    Differentiates malic acid from other compounds using mass spectrometry.

     

    3. Rapid Analysis

    Efficient sample preparation and fast detection processes for high-throughput analysis.

     

    4. High Accuracy

    Strict quality control ensures accuracy and reproducibility.

     

    5. Versatility

    Capable of analyzing malic acid and its related metabolites.

     

    Sample Submission Requirements

    1. Sample Types

    Serum, plasma, urine, tissue homogenates, and other biological samples.

     

    2. Sample Volume

    At least 200 μL of liquid sample or 200 mg of solid sample.

     

    3. Sample Preservation

    Store at -80°C to maintain stability.

     

    Note: Provide details on sample collection and handling.

     

    Applications

    • Detect malic acid content in food to ensure safety and quality.
    • Assess the intake of malic acid and its impact on health.
    • Investigate malic acid's role in metabolic diseases and other health conditions.
    • Study the applications and potential efficacy of malic acid in drug development and treatment.

     

    Deliverables

    1. Experimental Procedures

    2. Relevant Liquid Chromatography and Mass Spectrometry Parameters

    3. Detailed Information on Malic Acid

    4. Raw Data

    5. Custom Analysis Report

     

    MtoZ Biolabs is dedicated to advancing your research and clinical projects by providing detailed insights into malic acid's extensive physiological roles through top-tier analytical techniques and expert services.

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