Mass Spec Protein Sequencing Service

Mass Spec Protein Sequencing Service leverages advanced mass spectrometry technologies to provide precise and comprehensive analysis of protein sequences, structures, and post-translational modifications (PTMs). Unlike traditional sequencing methods, which struggle with large or modified proteins, mass spectrometry offers unparalleled sensitivity and speed, enabling the analysis of complex biological samples with high accuracy. By using techniques like MALDI-TOF and ESI-QTOF, MtoZ Biolabs delivers detailed protein characterization, allowing for the identification of novel proteins, mutations, and PTMs that are crucial for understanding biological functions and disease mechanisms.

 

This service has broad applications across various fields, including biomarker discovery, drug development, personalized medicine, and clinical diagnostics. In biomarker research, Mass spec protein sequencing can uncover novel protein biomarkers for early disease detection and therapeutic monitoring. In drug development, it facilitates the identification of protein targets and the evaluation of therapeutic candidates. Additionally, in clinical diagnostics, it aids in the detection of disease-related protein variants and mutations, contributing to more accurate diagnoses and personalized treatment plans.

 

Service at MtoZ Biolabs

At MtoZ Biolabs, we utilize state-of-the-art mass spec protein sequencing platforms to offer unparalleled services. Our team of seasoned scientists specializes in proteomics and is dedicated to addressing the challenges associated with MS-based sequencing. We provide comprehensive analytical solutions tailored to your research needs.

1. Protein N-Terminal Sequencing Service

We determine the amino acid sequence starting from the N-terminus of proteins. This service is vital for understanding protein maturation, processing events, and identifying signal peptides that influence protein targeting and function.

 

2. Protein C-Terminal Sequencing Service

Our C-terminal sequencing identifies the amino acid sequence at the protein's C-terminus. This information aids in studying protein stability, degradation pathways, and interaction sites essential for protein function.

 

3. Protein Full-Length Sequencing Service

MtoZ Biolabs provide comprehensive sequencing of entire protein molecules from end to end. Using mass spec protein sequencing allows us to achieve complete characterization of protein structures, facilitating novel protein discovery and the validation of recombinant proteins.

 

4. Protein De Novo Sequencing Service

Our de novo sequencing determines protein sequences without prior genetic or protein information. This is invaluable for characterizing unknown proteins, sequencing antibodies, and detecting post-translational modifications.

 

5. Top-Down Protein Sequencing Service

We analyze intact proteins using top-down mass spec protein sequencing techniques. This approach offers detailed insights into protein structure and post-translational modifications that may be overlooked by traditional methods.

 

Analysis Workflow

 



Figure 1. Workflow for Mass Spec Protein Sequencing

 

Service Advantages

1. Advance Analysis Platform: MtoZ Biolabs established an advanced Mass Spec Protein Sequencing Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all mass spec protein sequencing data providing clients with a comprehensive data report.

4. Fast Turnaround Times: Efficient workflows ensuring timely delivery of results.

 

Sample Submission Suggestions

1. Pure Protein: ≥20 micrograms

2. Complex Protein Mixtures: ≥50 micrograms

3. Cell Lysates: ≥100 micrograms

4. Tissue Samples: ≥10 milligrams

*Note: Please contact us if you have any special requirements or need assistance with your sample preparation.

 

Applications

1. Novel Protein Identification

Discover and characterize new proteins without prior sequence information, aiding in the expansion of proteomic databases and understanding of biological systems.

 

2. Post-Translational Modification Analysis

Mass Spec Protein Sequencing Service allows for precise detection of PTMs, such as phosphorylation, glycosylation, and acetylation, helping to unravel the regulatory mechanisms that influence protein activity.

 

3. Protein Variant and Mutation Detection

Mass spec protein sequencing can provide detailed insight into mutations and sequence variations, making it an essential tool for understanding genetic disorders.

 

4. Antibody Sequencing

Determine the amino acid sequences of antibodies for therapeutic development, vaccine design, and research into immune responses.

 

5. Biomarker Discovery

Mass Spec Protein Sequencing Service helps uncover protein biomarkers for early disease detection, prognosis, and monitoring therapeutic responses in clinical research.

 

6. Quality Control in Biopharmaceuticals

Verify the identity and purity of recombinant proteins and biologics, ensuring compliance with regulatory standards in drug development and manufacturing.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. Relevant Liquid Chromatography and Mass Spectrometry Parameters

4. The Detailed Information of Protein Sequencing Analysis 

5. Mass Spectrometry Image

6. Raw Data

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. If you are interested in our Mass Spec Protein Sequencing Service, please feel free to contact us.