Mass Spectrometry PRM Quantification Method
PRM (Parallel Reaction Monitoring) is a mass spectrometry quantitative technology, also known as "selective reaction monitoring". It is a highly sensitive, highly selective and highly accurate quantitative strategy based on third-order mass spectrometry.
In PRM (Parallel Reaction Monitoring) experiments, precursor ions of the target peptides are selectively introduced into a collision cell for activation, followed by parallel detection of all fragment ions. Conducted in a high-resolution, high-mass accuracy full-scan mode, PRM experiments provide richer quantitative and qualitative information compared to SRM, benefiting peptide identification and quantification. Moreover, PRM's consistent detection of all fragment ions throughout the experimental process enhances its quantitative repeatability and precision over SRM.
Methodology
1. Sample Preprocessing
This includes protein extraction, concentration determination, and digestion.
2. PRM Method Development
Determine the m/z values of the target peptides and set the collision energy parameters.
3. Sample Injection
Preprocessed samples are introduced into the mass spectrometer via high-performance liquid chromatography for analysis.
4. Data Analysis
Mass spectrometry data is collected and subjected to quantitative analysis using specialized software.
Advantages
1. PRM offers high sensitivity, selectivity, and precision, making it ideal for quantifying target proteins in complex samples.
2. Operating in full-scan mode, the PRM strategy captures all fragment ions, providing comprehensive quantitative and qualitative insights.
3. PRM experiments exhibit superior quantitative repeatability and precision.
Disadvantages
1. PRM requires advanced mass spectrometer performance and robust data processing capabilities compared to other mass spectrometry techniques.
2. Data analysis in PRM is complex and demands specialized software, which can be challenging.
Applications
PRM is extensively applied in biomedicine, environmental science, and food safety. It is instrumental in quantifying protein expression levels, investigating protein post-translational modifications, and discovering and validating biomarkers.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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