MtoZ Biolabs Advanced N-Terminal Sequencing Service

The N-terminal of a protein marks the beginning of its expression and is integral to regulating subcellular localization, degradation, and turnover. MtoZ Biolabs offers high-quality N-terminal sequencing service through an advanced platform that integrates Edman degradation with mass spectrometry, which is suitable for a variety of sample types, including but not limited to proteins, peptides, antibodies, vaccines, recombinant proteins, etc.

 

Why Choose MtoZ Biolabs

MtoZ Biolabs' N-terminal sequencing service combines Edman degradation and mass spectrometry to achieve precise N-terminal analysis. This approach effectively addresses scenarios where the N-terminal is blocked or modified (PTM), providing researchers with comprehensive analytical solutions.

 

1. Edman Sequencing

Delivers accurate N-terminal sequence information by sequentially cleaving amino acid residues from the protein's N-terminal.

 

2. Mass Spectrometry Analysis

Overcomes challenges related to N-terminal blocking and modifications (PTM), offering detailed sequence validation and information on modification sites.

 

In this service, protein sequence is not restricted by sample types, N-terminal blockage, and PTMs. Protein N-T sequences can be accurately analyzed by Edman sequencing, whereas, the N-terminal blockage and PTMs can be analyzed by mass spectrometry.

 

Experimental Instruments

1. SHIMADZU PPSQ-33A

2. Obitrap Fusion Lumos

 

Deliverables

1. Experimental Procedures

2. Parameters of Liquid Chromatography and Mass Spectrometry

3. MS Raw Data Files

4. Peptide Identification and Signal Intensity

5. Protein Identification and Signal Intensity