MtoZ Biolabs Targeted Amino Acid Metabolomics: Precision Quantification through Advanced MRM Technology
- Absolute quantification using isotopically labeled internal standards.
- High sensitivity for low-abundance metabolites.
- Comprehensive data analysis for generating accurate concentration profiles of amino acids and derivatives across multiple sample types.
Amino acids and their derivatives play crucial roles in various biological functions, serving as the fundamental units of proteins and participating in critical metabolic pathways such as neurotransmission, immune response, and energy production. The precise quantification of amino acids and their related compounds in biological samples is essential for understanding their roles in health and disease. To meet the growing demand for high-precision metabolomics research, MtoZ Biolabs offers a specialized MRM-based targeted metabolomics service focused on the analysis of amino acids and their derivatives.
MtoZ Biolabs Targeted Amino Acid Metabolomics Project Overview
The targeted metabolomics approach provides a comprehensive analysis of amino acids, utilizing advanced Multiple Reaction Monitoring (MRM) technology. This service allows for the precise and simultaneous quantification of amino acids and their derivatives in complex biological samples. The MRM method is known for its high specificity, sensitivity, and accuracy, making it ideal for detecting metabolites present in low concentrations. Our platform is optimized for various sample types, ensuring accurate and reproducible results across a wide range of applications, from clinical studies to basic research.
MRM Principle
Our analysis is based on SRM/MRM (Selected/Multiple Reaction Monitoring) technology, which uses isotopically labeled standards as references. MRM achieves exceptional specificity by monitoring precursor-product ion transitions, enabling the precise identification and quantification of targeted metabolites in a complex matrix. The high sensitivity of MRM allows for the detection of metabolites even in minute concentrations, with the added advantage of improved signal-to-noise ratios, reduced interference, and enhanced detection limits.
The MRM method utilizes tandem mass spectrometry (MS/MS) to monitor specific ion transitions, which ensures accurate detection of target compounds. This technology allows for both qualitative and quantitative analysis of the metabolites of interest with exceptional accuracy.
Experimental Process
The experimental workflow follows a systematic process to ensure high-quality results:
1. Sample Preparation
Biological samples, such as plasma, serum, tissues, or cells, are collected and prepared for analysis. Samples are typically prepared through protein precipitation, centrifugation, and subsequent storage at optimal conditions (-80°C).
2. LC-MS/MS Analysis
The prepared samples are analyzed using Agilent 1290 Infinity LC coupled with 5500 QTRAP mass spectrometry (AB SCIEX). Chromatographic separation is achieved using a reversed-phase column with gradient elution. The samples undergo ionization via ESI (Electrospray Ionization) under controlled temperature and gas flow conditions, ensuring accurate ion generation.
3. Quantification and Data Acquisition
During analysis, our advanced MRM method captures the transitions of the analytes of interest, providing a detailed profile of amino acid concentrations. The data is processed using MultiQuant software, which allows for accurate quantification and correction of sample variations.
Data Processing and Analysis
Data from the MRM analysis is carefully processed using advanced software tools. The method ensures:
Service Advantages
✔️ High Sensitivity and Specificity: With MRM technology, even trace amounts of amino acids and derivatives can be detected.
✔️ Robust Quantification: The use of internal standards ensures precise, reproducible, and accurate quantification of target analytes.
✔️ Wide Sample Compatibility: We accommodate various biological matrices, including plasma, serum, tissue, and cell extracts.
✔️ Efficient Turnaround: Our streamlined workflow and advanced instrumentation allow for rapid data acquisition and analysis.
Applications
The targeted amino acid and derivatives analysis is critical in various research areas, including:
✔️ Clinical Diagnostics: For biomarker discovery and validation in metabolic disorders.
✔️ Nutritional Research: Studying the impact of amino acid metabolism on overall health and nutrition.
✔️ Pharmacology: Understanding the influence of drugs on amino acid metabolism.
✔️ Basic Research: Investigating amino acid roles in cell signaling, autophagy, and immune response.
By offering a targeted approach to amino acid metabolomics, MtoZ Biolabs is positioned to provide unparalleled support for researchers seeking to gain insights into the complex dynamics of amino acid metabolism. This service is ideal for those looking to advance their research with high-precision, data-driven results.
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