Multiple Reaction Monitoring Analysis Service

The multiple reaction monitoring analysis service is designed to provide highly precise quantification of target molecules in complex samples using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS). As life sciences research advances, the demand for accurate quantification in drug development, biomarker validation, and metabolomics research continues to grow.

 



Guerrero, L. et al. Metabolites. 2022.

Figure1. Multiple Reaction Monitoring Analysis Principle

 

Multiple reaction monitoring technology achieves precise quantification of target molecules by monitoring specific reactions between precursor and product ions in tandem mass spectrometry. The first mass analyzer selects precursor ions, which are fragmented in the collision cell to generate product ions. The second mass analyzer then isolates the target product ions, ensuring high specificity. Combined with optimized chromatographic separation, Multiple Reaction Monitoring effectively minimizes matrix interference, achieving ultra-high sensitivity and a broad dynamic range in quantitative analysis. The efficiency and reliability of Multiple Reaction Monitoring have made it an indispensable tool in modern molecular research.

 

Service at MtoZ Biolabs

Leveraging cutting-edge instruments, including the Thermo Scientific Orbitrap Fusion Lumos mass spectrometer and Nano-LC system, along with a professional technical team and extensive project experience, MtoZ Biolabs offers precise multiple reaction monitoring analysis service, encompassing the optimization of targeted detection methods, development and validation of quantification strategies, and result interpretation using advanced algorithms and databases. By choosing our services, you gain access to top-tier technical support and a trusted partner to explore the frontiers of scientific research.

 



 

 

Applications

As a highly sensitive and specific quantitative mass spectrometry technique, multiple reaction monitoring demonstrates extensive utility in research. From elucidating differences in protein expression to uncovering dynamic changes in metabolic networks, multiple reaction monitoring stands out for its reliability and precision. MtoZ Biolabs' multiple reaction monitoring analysis service offers robust quantitative support across diverse applications, including biomarker validation, metabolite detection, drug metabolism studies, and post-translational modification (PTM) analysis. We provide customized solutions to help researchers navigate complex biological systems and drive scientific discoveries toward publication and practical application.

 

1. Protein Quantification

Multiple reaction monitoring enables absolute quantification of proteins with high sensitivity, specificity, and reproducibility. By monitoring the precursor and product ions of specific peptides, researchers can:

• Validate potential biomarkers, such as tumor-associated or disease-progression proteins.
• Compare protein expression levels in healthy versus diseased states.
• Track therapeutic targets, such as key proteins in drug mechanisms of action.

 

2. Metabolite Detection and Quantification

Multiple reaction monitoring is extensively utilized in metabolomics research, particularly for:

• Detection and quantification of endogenous metabolites (e.g., amino acids, fatty acids, carbohydrates) to investigate metabolic pathways.
• Analysis of exogenous compounds (e.g., drugs and their metabolites) for pharmacokinetics (PK) and toxicity studies.

 

3. Drug Metabolism and Pharmacokinetics (PK) Studies

Multiple reaction monitoring is a preferred tool for analyzing drug concentrations and metabolites due to its high sensitivity and specificity:

• Measure drug plasma concentrations at various time points to support PK modeling.
• Track drug metabolism pathways, including formation and clearance in vivo.
• Investigate drug-drug interactions by evaluating metabolic pathway alterations under co-administration.

 

4. Post-Translational Modification (PTM) Protein Quantification

Multiple reaction monitoring can quantify PTMs, such as phosphorylation, methylation, or acetylation:

• Study signaling pathways by quantifying phosphorylated proteins to decode cell signaling mechanisms.
• Compare dynamic changes in modification states under different stimuli.

 

5. Biomarker Validation and Translation

Multiple reaction monitoring is frequently used to validate candidate biomarkers, bridging the gap from discovery to clinical application:

• Confirm the relevance of proteins or metabolites to disease.
• Screen biomarkers for disease diagnosis, classification, or prognosis.

 

6. Target Molecule Detection in Complex Biological Samples

Multiple reaction monitoring excels in detecting specific molecules in complex biological matrices, such as plasma, urine, and tissue extracts:

• Monitor environmental exposure markers, such as pollutants or toxins.
• Conduct multi-component analyses for simultaneous detection of multiple target compounds, ideal for studying complex disease mechanisms.

 

FAQs

 

1. How to select suitable target peptides to ensure multiple reaction monitoring analysis specificity and sensitivity?

 

Multiple reaction monitoring analysis relies on precise target peptide selection, considering factors like ionization efficiency, specificity, and potential interference from PTMs or noise. Combining database predictions with experimental validation can optimize peptide selection, ensuring reliable results.

 

2. How to optimize precursor and product ion pairs to improve multiple reaction monitoring quantification accuracy?

The key to multiple reaction monitoring analysis lies in selecting optimal precursor and product ion pairs. By fine-tuning collision energy and adjusting mass spectrometer parameters, researchers can maximize the signal-to-noise ratio and minimize matrix interference, enhancing both sensitivity and specificity.

 

MtoZ Biolabs offers precise multiple reaction monitoring analysis service for highly sensitive and specific quantification of target proteins, peptides, and metabolites. With a strong track record in biomarker validation, pharmacokinetics, and PTM research, we deliver tailored solutions to meet your research needs. Partner with us to achieve accurate, reliable, and publishable results that drive breakthroughs in your scientific projects.