N-Glycosylation Site Analysis Service | Biological Products

N-glycosylation is a prevalent and essential post-translational modification of proteins, characterized by the covalent attachment of sugar moieties to specific asparagine (Asn) residues. This modification plays a critical role in influencing protein folding, stability, intracellular transport, and functional activity. In the biopharmaceutical field, N-glycosylation significantly affects the physicochemical properties of proteins and directly impacts drug efficacy, safety, and immunogenicity. Therefore, a thorough understanding of N-glycosylation sites and their modification patterns is crucial for elucidating disease mechanisms, optimizing biopharmaceutical processes, and enhancing product quality.

 

The N-Glycosylation Site Analysis Service offered by MtoZ Biolabs is designed to help clients comprehensively characterize the glycosylation status of target proteins, providing precise data support for the development and quality control of biopharmaceuticals.

 

Analysis Workflow

To determine glycosylation attachment site, protein N-glycosidase F (PNGase F) is typically used in heavy water to separate polysaccharides from proteins. During this process, the originally glycosylated asparagine undergoes deamidation to become aspartic acid, with an addition of an ¹⁸O modification. Because the introduction of ¹⁸O changes the molecular weight, glycosylation sites can be detected by comparing peptides containing aspartic acid with those containing unglycosylated asparagine. The general strategy for analyzing variable N-glycosylation site in glycoproteins includes protein digestion with PNGase F, polysaccharide release, separation of glycopeptide by liquid chromatography (LC), and detection by mass spectrometry (MS).

 



 

Service Advantages

1. High Sensitivity and Resolution

HPLC-MS provides superior resolution and mass accuracy for detecting low-abundance N-glycosylation modifications.

 

2. Precise Identification and Quantification

Isotopic labeling combined with mass spectrometry allows for accurate identification and quantification of each N-glycosylation site.

 

3. Comprehensive Analysis

Suitable for the analysis of diverse N-glycosylation modifications in complex biological samples, offering extensive glycosylation state information.

 

4. Customized Solutions

Tailored analysis plans are provided based on customer requirements, accommodating different sample types and research goals.

 

Applications

1. Biopharmaceutical Development and Optimization:Analysis and optimization of N-glycosylation patterns in biopharmaceuticals (e.g., monoclonal antibodies, recombinant protein drugs) to enhance efficacy and stability.

 

2. Quality Control and Regulatory Compliance: Evaluation of batch-to-batch consistency and quality control during biopharmaceutical production to ensure regulatory compliance.

 

3. Biomarker Discovery: Identification and validation of N-glycosylation modifications associated with specific diseases for diagnostic biomarker discovery.

 

Deliverables

1. Experimental Procedures

2. Mass Spectrometric Parameters

3. Mass Spectrometric Images

4. Raw Data

5. Detailed Information on Identified Glycosylation Sites

 

MtoZ Biolabs has much analytical service experience in glycomics and can customize glycomics analysis services according to your specific needs. Free consultation is available!