N6,N6,2'-O-trimethyladenosine Analysis Service
- Extensive expertise in handling various biological samples and extracting metabolites.
- State-of-the-art mass spectrometry platforms.
- Strict quality control to ensure reliable results.
- Comprehensive statistical and bioinformatics analysis.
- Personalized service to meet various research needs.
- Biomarker discovery
- Drug development
- Disease diagnosis and prognosis
- Animal tissue ≥ 100 mg
- Plant tissue ≥ 200 mg
- Serum/ Plasma ≥ 200 μL
- Urine ≥ 2 mL
N6,N6,2'-O-trimethyladenosine (m62Am) is a unique and highly methylated nucleoside found in various RNA molecules, particularly transfer RNA (tRNA) and messenger RNA (mRNA). Structurally, m62Am features two methyl groups attached to the nitrogen atoms at the N6 position of adenine, along with an additional methyl group on the 2'-hydroxyl of the ribose sugar. This extensive methylation significantly alters the chemical properties of the nucleoside. It plays a crucial role in stabilizing RNA structure, enhancing translational efficiency, and modulating interactions between RNA and protein. Aberrant methylation patterns of nucleosides like m62Am are increasingly being recognized as biomarkers for disease progression.
Figure 1. The Structure of N6,N6,2'-O-trimethyladenosine
The biological role of m62Am is still under investigation, but current studies suggest that this modification plays an important part in the fine-tuning of gene expression, particularly during translation. In tRNA, it is thought to stabilize the anticodon loop, improving codon recognition and enhancing translation accuracy. In mRNA, the modification may contribute to the regulation of mRNA stability, splicing, and translational control. Additionally, m62Am is implicated in cellular responses to environmental stress and has been associated with various biological processes, including cellular differentiation and metabolism. Its potential as a regulatory element in RNA biology makes it a valuable target for research in fields such as epitranscriptomics, molecular biology, and disease pathology.
To support research in this important area, MtoZ Biolabs offers an advanced N6,N6,2'-O-trimethyladenosine Analysis Service, leveraging cutting-edge mass spectrometry technologies to deliver accurate and sensitive analysis of m62Am in various biological samples, enabling researchers to gain critical insights into its biological roles and implications in health and disease.
Analysis Workflow
Service Advantages
Applications
Our N6,N6,2'-O-trimethyladenosine analysis service can be applied in various fields, including but not limited to:
Sample Submission Requirements
1. Sample Types
Serum, plasma, urine, tissues and other biological samples. It is necessary to select more than 3 materials with the same condition for each sample.
2. Sample Volume
3. Sample Preservation
Store at -80°C to maintain stability.
Note: Provide details on sample collection and handling.
Deliverables
1. Experimental Procedures
2. Relevant Liquid Chromatography and Mass Spectrometry Parameters
3. Detailed Information on N6,N6,2'-O-trimethyladenosine
4. Raw Data
5. Custom Analysis Report
Experience the best N6,N6,2'-O-trimethyladenosine analysis service at MtoZ Biolabs. Contact us now for more information.
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