NMR Analysis of Peptide Drugs

Polypeptide drugs are large biomolecules formed by the connection of dozens to hundreds of amino acids via peptide bonds. Their biological activity and stability depend on their three-dimensional structure, making the analysis of their structure crucially important. Nuclear Magnetic Resonance (NMR) is a powerful analysis technique that can provide detailed structural information about polypeptides, including their atom-level three-dimensional structure, dynamic behavior, and interactions with other molecules.

 

The principle of NMR is based on the behavior of magnetic nuclei (such as hydrogen atoms) in a magnetic field. When placed in a magnetic field, these nuclei produce subtle frequency differences according to their different chemical environments, which can be detected by NMR instruments. By analyzing these frequency differences (known as chemical shifts), the environment and relative position of atoms can be inferred, thus providing information about the molecular structure. With multidimensional NMR techniques, detailed studies can be conducted on large molecules and complex systems.

 

In the research of polypeptide drugs, NMR is often used to determine the three-dimensional structure of polypeptides, study the interactions of polypeptides with receptors or other molecules, and explore the dynamic behavior of polypeptides. Through this information, scientists can understand the mechanism of action of polypeptide drugs, optimize their design, improve their stability and biological activity, to meet therapeutic needs. Although NMR requires professional equipment and technical knowledge, its value for understanding and improving polypeptide drugs is invaluable.

 

MtoZ Biolabs provides customers with drug quality research services that meet global drug regulatory laws. MtoZ Biolabs combines GPC separation method for Nuclear Magnetic Resonance Spectroscopy (NMR) analysis to meet your needs for sequencing and structural analysis of polypeptide drugs. The polymer analysis service based on Nuclear Magnetic Resonance Spectroscopy (NMR) is combined with the GPC separation method to provide you with the NMR detection platform, helping you characterize and develop polypeptide drugs.