Nuclear Magnetic Resonance Analysis of Recombinant Protein Vaccines

Recombinant protein vaccines are a type of vaccine that do not contain intact pathogens and are prepared from specific protein antigens produced by heterologous expression systems. Common heterologous expression systems include: bacteria, mammalian cells, plant cells, and insect cells, and often need to choose the appropriate heterologous expression system according to the type of antigen produced. Recombinant protein vaccines have the advantages of good safety, strong stability, and low cost, and have been widely favored by researchers in recent years. Currently, recombinant protein vaccines have been widely used in various infectious diseases, such as preventive hepatitis B virus (HBV), tetanus, whooping cough, influenza, etc. In the study of recombinant protein vaccines, dissecting the structure is very important for understanding its pharmacological activity, stability, transmission pathway, and toxicity.

 

Nuclear Magnetic Resonance (NMR) analysis is an important research method for the structure of recombinant protein vaccines. This technology is also a main analysis technology of structural information of organic compounds and biomacromolecules, as it can provide valuable structural information of the whole molecule through a series of analysis tests in many cases. The range of NMR spectral analysis objects ranges from organic small molecules or metabolites to medium-sized peptides or natural products, and then to biomacromolecules and synthetic high-molecular-weight polymers. As a powerful analysis platform, NMR spectroscopy can replace other techniques (such as X-ray, crystallography, and mass spectrometry) to obtain structural information.

 

MtoZ Biolabs provides drug quality research services that comply with global pharmaceutical regulations. By combining GPC separation methods with Nuclear Magnetic Resonance (NMR) spectroscopy, we meet your needs for recombinant protein vaccine sequencing and structural analysis. Our polymer analysis services, based on NMR spectroscopy and integrated with GPC separation methods, offer an NMR testing platform that supports you in the characterization and development of recombinant protein vaccines.