Nucleotide Metabolism Service

Nucleotides, the fundamental building blocks of nucleic acids, play a critical role in various biological processes. Each nucleotide consists of a pentose sugar, a phosphate group, and a nitrogenous base, which can be either a purine (adenine and guanine) or a pyrimidine (cytosine, uracil, and thymine). These molecules form the backbone of DNA and RNA, carrying genetic information crucial for cell function and heredity.

 
In addition to their role in genetics, nucleotides are involved in cellular energy transfer, signaling pathways, and enzymatic reactions. For instance, adenosine triphosphate (ATP) and guanosine triphosphate (GTP) serve as primary energy currencies of the cell, driving myriad biochemical processes. Moreover, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate function as secondary messengers in signal transduction pathways, highlighting the versatility and importance of nucleotides in cellular communication and metabolism. As a part of metabolic pathways, nucleotides can be synthesized, broken down, or recycled based on the cell's needs. Understanding the intricate balance and regulation of nucleotide metabolism is key to unraveling the molecular mechanisms of various diseases and exploring potential therapeutic strategies.
 

 
Figure 1. The Structure of Nucleotide
 
 

At MtoZ Biolabs, we offer a comprehensive nucleotide metabolism service that leverages our state-of-the-art mass spectrometry technologies for the sensitive and specific detection and quantification of nucleotides. Utilizing high-performance liquid chromatography-mass spectrometry (HPLC-MS), our experienced scientists conduct both targeted and untargeted analyses to provide detailed profiling of nucleotide metabolic pathways, ensuring high-quality, reliable, and reproducible results. This service empowers researchers with the insights needed to explore nucleotide functions and interactions within biological systems.

 

Analysis Workflow

 



 

Service Advantages

• Advanced HPLC-MS equipment with high sensitivity and accuracy
• Comprehensive data analysis supported by a team of experienced analysts
• Strict quality control to ensure reliable results
• Personalized service to meet various research needs

 

Applications

Our nucleotide metabolism service can be applied in various research areas, including but not limited to:

• Disease mechanism research
• Drug discovery and development
• Biomarker discovery
• Nutritional and metabolic studies

 

Sample Submission Requirements

• Type of samples: Plasma, serum, cell, tissue, etc.
• Quantity: At least 200 μl liquid, 1 x 10⁷ cells, or 200 mg tissue.
• Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
• Note: Avoid repeated cycles of freezing and thawing

For more details, please feel free to get in touch with our customer service team.

 

Deliverables

1. Experimental Procedures

2. Relevant High-Performance Liquid Chromatography and Mass Spectrometry Parameters

3. Detailed Information on Nucleotide Metabolism Analysis

4. Raw Data

5. Custom Analysis Report

 

At MtoZ Biolabs, we are committed to providing you with the most comprehensive, accurate, and reliable nucleotide metabolism service to support your research. Contact us today to learn more about how we can assist you with your nucleotide metabolism studies.