Glycan Profiling

Glycosylation is integral to numerous biological processes, including protein folding, cell-cell recognition, signal transduction, and immune responses. Aberrant glycosylation patterns are linked to diseases such as cancer, infectious diseases, and diabetes. Glycan Profiling refers to the qualitative and quantitative analysis of glycans on proteins, lipids, or other biomolecules. This profiling provides insights into glycan structures, compositions, and their alterations. Glycosylation analysis is crucial for the quality control and functional evaluation of biopharmaceuticals like monoclonal antibodies. Additionally, glycosylation patterns serve as biomarkers for disease diagnosis and treatment monitoring.

 

MtoZ Biolabs has established a robust and professional Glycan Profiling platform, utilizing the Thermo Fisher Q Exactive HF and Orbitrap Fusion Lumos mass analyzer systems, coupled with a Nano-LC system. Our objective is to offer unparalleled support for our clients' research.

 

Analysis Workflow

1. Sample Preparation: Includes protein extraction, glycan release, and labeling.

 

2. Separation and Purification: Glycans are separated and purified using HPLC, CE, and other methods.

 

3. Detection and Identification: Glycans are detected and identified via MS, HPLC, CE, and other techniques.

 

4. Data Analysis: Detection results are analyzed to obtain glycan structures and quantitative information.

 



Figure 1. A General Scheme for Glycan Profiling

 

Service Advantages

1. High Sensitivity and Specificity: The integration of mass spectrometry and liquid chromatography ensures highly sensitive and specific analyses.

 

2. Diverse Analytical Capabilities: Capable of analyzing various glycan structures in complex samples.

 

3. Quantitative Analysis: Provides both relative and absolute quantitative information on glycans.

 

4. High Throughput: Capable of simultaneously analyzing multiple samples, enhancing efficiency.

 

Applications

1. Understanding Biological Processes: Elucidates the role of glycosylation in cellular biology and pathophysiology. 

 

2. Disease Diagnosis and Monitoring: Tracks disease progression and therapeutic effects through changes in glycosylation patterns.

 

3. Drug Quality Control: Ensures biopharmaceutical glycosylation patterns meet standards, guaranteeing drug safety and efficacy.

 

4. Discovering New Targets: Identifies new therapeutic targets and diagnostic biomarkers through glycosylation analysis.

 

MtoZ Biolabs' Glycan Profiling service provides comprehensive insights into glycosylation roles in various biological and pathological processes, delivering critical scientific and clinical information. This service shortens experimental cycles and addresses research challenges. For consultations, please contact us.