Glycosylation site analysis

Glycosylation sites are specific amino acid residues on protein molecules, typically asparagine (Asn) or serine/threonine (Ser/Thr), where glycosylation modifications occur. Glycosylation involves the covalent attachment of sugar chains to proteins, constituting an important post-translational modification (PTM). Analyzing glycosylation sites provides insights into the locations of these sites, the structures of the attached sugar chains, the extent of glycosylation, and its effects on protein function and stability, as well as various biological processes. Consequently, glycosylation site analysis is crucial in disease diagnosis, drug development, and protein function research.

 

MtoZ Biolabs offers glycosylation site analysis services, aiding researchers in identifying and characterizing glycosylation modifications, assessing protein quality and purity, and exploring the role of glycosylation in diseases. For more information on our services, please contact us.

 

Analysis Workflow

1. Enzymatic Digestion: Specific proteases (such as trypsin) are used to cleave proteins into peptides.

 

2. Enrichment of Glycopeptides: Specialized methods (such as lectin affinity chromatography or solid-phase extraction) are employed to enrich peptides containing glycosylation sites.

 

3. Mass Spectrometry Analysis: Enriched glycopeptides are analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) to identify glycosylation sites and sugar chain structures.

 

4. Data Analysis: Specialized software and databases (such as GlycoMod) are utilized for data interpretation to determine glycosylation sites.

 



An, HJ. et al. Curr Opin Chem Biol. 2009.

Figure 1. The Standard Approaches to Determine Site-Specific Glycosylation

 

Service Advantages

1. High Sensitivity: Mass spectrometry can detect trace amounts of glycopeptides.

 

2. High Resolution: Accurately locates glycosylation sites and identifies sugar chain structures.

 

3. High Throughput: Enables simultaneous analysis of multiple samples and sites.

 

4. Comprehensive Information: Provides detailed data on glycosylation sites, sugar chain structures, and glycosylation levels.

 

Sample Submission Requirements

 



For more sample details, please consult our technical team.

 

Applications

1. Identification of Disease Biomarkers: Analysis of glycosylation patterns to discover biomarkers associated with diseases.

 

2. Drug Development and Quality Control: Optimization of glycosylation patterns in biopharmaceuticals to ensure drug quality.

 

3. Basic Research: Elucidation of the role of glycosylation in protein function and cell biology.

 

4. Diagnosis and Treatment: Development of diagnostic tools and therapeutic approaches based on glycosylation sites.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Glycosylation Site

4. Mass Spectrometry Images

5. Raw Data