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    Phosphoenolpyruvate Analysis Service

      Phosphoenolpyruvate (PEP) is a carboxylic acid that forms through the transformation of pyruvate’s enol form, combined with phosphate. It primarily exists in its anionic form in biological systems. It is a key metabolite involved in many biochemical pathways, including glycolysis and gluconeogenesis.  As a crucial biochemical intermediate, PEP possesses one of the most high-energy phosphate bonds in organisms. The detailed analysis of PEP is essential for elucidating the mechanisms underlying various metabolic disorders, cancer, and other diseases, providing insights that are vital for advancing medical research and therapeutic strategies.

       

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      Figure 1. The Structure of Phosphoenolpyruvate

       

      Services at MtoZ Biolabs

      At MtoZ Biolabs, our Phosphoenolpyruvate Analysis Service is based on advanced liquid chromatography-tandem mass spectrometry (LC-MS/MS). The procedure begins with the extraction and purification of metabolites from samples. The purified metabolites are then separated by LC and detected by MS/MS. The data obtained from the MS/MS detection are processed and analyzed to provide accurate quantification of Phosphoenolpyruvate.

       

      Analysis Workflow

       

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      Service Advantages

       

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      Applications

      Our Phosphoenolpyruvate Analysis Service can be applied in various research areas including but not limited to:

      • Metabolic disorder studies
      • Cancer research
      • Drug development
      • Biomarker discovery

       

      Sample Submission Requirements

      • Please ensure your submitted sample surpasses 0.2 g or 0.2 mL.
      • Samples should be stored in a -80℃ environment and transported on dry ice.

       

      Deliverables

      1. Experimental Procedures

      2. Relevant Mass Spectrometry Parameters

      3. Detailed Information on Phosphoenolpyruvate Analysis

      4. Mass Spectrometry Images

      5. Raw Data

       

      At MtoZ Biolabs, we are committed to providing you with the most reliable and accurate Phosphoenolpyruvate Analysis Service to assist in your research. Contact us today to learn more about our services or to start your project.

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