Phosphorylation Site and Abundance Detection
Phosphorylation is a critical post-translational modification (PTM) of proteins, integral to several biological processes such as signal transduction, cell division, and metabolism. This process involves the addition of a phosphate group to specific amino acid residues, typically serine (S), threonine (T), and tyrosine (Y).
Detection of Phosphorylation Sites
The precise identification of phosphorylation sites on proteins is crucial for understanding their functional roles. Mass spectrometry is commonly employed for this purpose, providing detailed mass spectra that reveal phosphorylation events and pinpoint specific sites. Additionally, bioinformatics tools, including various predictive algorithms, are utilized to forecast potential phosphorylation sites in protein sequences.
Measurement of Phosphorylation Abundance
Assessing the relative abundance of phosphorylated proteins within a sample is important for elucidating their role in biological processes. Techniques such as Western blotting with phosphorylation-specific antibodies and immunoprecipitation are often used to quantify phosphorylation levels.
Accurate detection and quantification of phosphorylation sites and their abundance enhance our understanding of protein phosphorylation's impact on biological functions.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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