Phytosulfokine (PSK) Analysis Service

 

Phytosulfokine (PSK) is a plant-derived peptide hormone that promotes cell division and enhances plant resistance to stress. As an important growth regulator, PSK plays a key role in plant development and environmental stress response. Accurate analysis of PSK content and activity is crucial for plant biotechnology, agricultural science research, and ecological monitoring. MtoZ Biolabs offers a Phytosulfokine (PSK) analysis service using advanced mass spectrometry techniques and bioactivity assays to ensure precise and reliable data for research and application.

 



Suri, G.S. et al. Int. J. Plant Biol. 2023.

Figure 1. The Role of PSKs in Nodule Organogenesis

 

PSK typically involves two main steps: mass spectrometric analysis and bioactivity assays. Through LC-MS/MS, we can accurately quantify the PSK content in samples, while specific bioassays, such as cell proliferation tests, assess its bioactivity. This integrated analytical approach not only provides quantitative data of PSK but also reveals its functional effects in organisms.

 

Analysis Workflow

• Sample Submission: Clients submit plant tissue or cell culture samples according to provided guidelines.
• Sample Pre-treatment: Includes crushing, extraction, and purification of plant tissues to suit subsequent LC-MS/MS analysis.
• LC-MS/MS Analysis: Samples are separated using a high-performance liquid chromatography system, followed by precise identification and quantification through tandem mass spectrometry.
• Bioactivity Assay: The growth regulatory activity of PSK is evaluated through specific cellular experiments.
• Data Analysis and Reporting: Provides a detailed report including PSK concentration, bioactivity, and its physiological impact.
• Technical Support and Consultation: Offers professional data interpretation and recommendations for further research.

 



 

Service Advantages

• Highly Efficient Sample Recovery and Processing
• Accurate and Reliable Detection and Quantification
• Capability to Handle Various Sample Types
• Customized Data Analysis Services
• Rapid Service Response and Cost-Effectiveness

 

Sample Submission Requirements

We can analyze a variety of biological materials, including but not limited to plant cells and tissues. If you need to ship your samples to us, please follow the requirements for different types of samples:

 

• Plant tissue: 200mg/sample
• Cells: 1x107/sample
• Shipping conditions: Dry ice

 

Deliverables

1. Experimental scheme

2. Instrument factors of MS

3. Raw data files of MS and MS data quality summary

4. Bioinformatics analysis report

 

With our advanced PSK analysis LC-MS platform, professional bioinformatics analysis software, and expert technical staff and scientists, MtoZ Biolabs provides customized PSK analysis services. Our services feature quick experimental procedures and easy-to-read reports, designed to accelerate your scientific research.