Plant Stem Vein Protein Isoelectric Point Analysis Based on cIEF

Plant stem vein capillary isoelectric focusing (cIEF) based protein isoelectric point analysis is a sophisticated biotechnology, specifically designed for exploring the isoelectric point (pI) of proteins in plants. This technology is especially important in plant science research, not only revealing the molecular characteristics of proteins, but also helping researchers to deeply understand plant physiological processes, mechanisms of response to environmental stress, and protein expression changes under pathological conditions.

 

Technical Principle

Capillary isoelectric focusing (cIEF) is a highly efficient protein isoelectric point analysis technology. It establishes a pH gradient in the capillary and applies an electric field, causing the protein to focus into sharp bands at its isoelectric point. In this technology, the protein moves to the position in the capillary where the pH value matches its isoelectric point and stops moving, thereby realizing the separation of different proteins. The high resolution and high sensitivity of cIEF technology make it an ideal choice for studying protein isoelectric points.

 

Service Advantages

1. High Resolution

cIEF can distinguish proteins with very close isoelectric points, providing high-precision analysis results.

 

2. High Sensitivity

Even protein samples of extremely low concentrations can be detected and analyzed, suitable for precious or hard-to-obtain samples.

 

3. Rapid Analysis

Compared with traditional isoelectric focusing electrophoresis, cIEF has a faster analysis speed and can complete protein isoelectric point analysis in a short time.

 

4. Wide Range of Applications

It is suitable for plant stem vein proteins from different sources, including transgenic plants and plants in their natural state.

 

Applications

1. Plant Physiological Research

Through analyzing the isoelectric point of stem vein proteins, the regulatory mechanisms of plants towards photosynthesis, nutrient absorption and other physiological functions are deeply understood.

 

2. Environmental Stress Response

Evaluate the changes in protein expression in plants under environmental stresses such as drought and salt stress, and reveal the stress response mechanism of plants.

 

3. Pathological State Analysis

Identify proteins specifically expressed in the disease state of plants, providing a molecular-level understanding for disease prevention and control.

 

4. Variety Improvement and Breeding

Screen plant stems with specific protein expression characteristics to guide the improvement and breeding of plant varieties.

 

MtoZ Biolabs has established a isoelectric point detection platform for different protein samples, in addition, we can also provide complete services including method establishment, verification and subsequent sample detection. Free project evaluation,Welcome to learn more details!