Plasma Proteomic Analysis Methods

Plasma, the concentrated essence of the fluid in our bodies, contains more than 10,000 proteins. Fortunately, thanks to assiduously studied plasma proteomics, we can now peer into these microscopic worlds at incredibly fine levels of detail. But how to choose the appropriate method for plasma proteomic analysis?

 

Proteins are the primary carriers of life activities and are the fundamental building blocks of our life. The proteins in plasma reflect physiological and pathological states, and through the analysis of plasma proteomics, we can decipher the health indices of life.

 

Common Methods of Plasma Proteomic Analysis

1. Liquid Chromatography-Mass Spectrometry (LC-MS/MS)

This is currently the most widely used proteomic analysis technology. This technique uses ion intensity to quantify proteins, has high accuracy and a low false-positive rate.

 

2. Two-Dimensional Electrophoresis-Mass Spectrometry

This method first uses two-dimensional electrophoresis to separate proteins on a gel, and then selects different protein spots for mass spectrometry analysis, which is more suitable for proteomic analysis of small sample volumes.

 

When selecting a method, factors such as the source, quantity, and complexity of the sample should be considered. Liquid chromatography-mass spectrometry is suitable for handling large amounts of complex samples, while two-dimensional electrophoresis-mass spectrometry is suitable for processing small sample volumes and relatively simple samples.