• Home
  • Biopharmaceutical Research Services
  • Multi-Omics Services
  • Support
  • /assets/images/icon/icon-email-2.png

    Email:

    info@MtoZ-Biolabs.com

    Preparation of Single Cell Suspensions for Mass Cytometry

      Materials and Instruments

      1. Experimental cell lines

      2. Phosphate-buffered saline (PBS)

      3. 0.25% Trypsin-EDTA

      4. Centrifuge

      5. Flow cytometer

       

      Preparation Procedure

      1. Cell Washing and Harvesting

      Begin by washing the cells with PBS. Following this, treat the cells with 0.25% Trypsin-EDTA to facilitate cell detachment and harvesting.

       

      2. Suspension and Centrifugation

      Resuspend the detached cells in PBS. Transfer the suspension to a centrifuge tube, and centrifuge at 300g for 5 minutes to pellet the cells.

       

      3. Resuspension and Filtration

      After discarding the supernatant, resuspend the cell pellet in fresh PBS. Filter the cell suspension to remove any aggregates and large debris.

       

      4. Cell Counting and Dilution

      Use a cell counter or hemocytometer to determine the cell concentration. Adjust the cell suspension to the desired dilution according to experimental requirements.

       

      5. Preparation for Mass Spectrometry Analysis

      Prepare a single-cell suspension tailored for mass spectrometry analysis. This step may require modifications based on the specific protocols associated with the mass spectrometer in use.

       

      MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.

      Related Services

      Single Cell Mass Cytometry Analysis

    Submit Inquiry
    Name *
    Email Address *
    Phone Number
    Inquiry Project
    Project Description *

     

    How to order?


    /assets/images/icon/icon-message.png

    Submit Inquiry

    /assets/images/icon/icon-return.png