Primary Bile Acid Biosynthesis Analysis Service
- Advanced HPLC-MS equipment with high sensitivity and accuracy
- Comprehensive data analysis supported by a team of experienced analysts
- Strict quality control to ensure reliable results
- Personalized service to meet various research needs
- Clinical Research – Understanding the role of primary bile acids in diseases like PFIC and metabolic disorders.
- Drug Discovery – Evaluating the impact of potential drugs on primary bile acid biosynthesis.
- Metabolic Studies – Studying the metabolic pathways associated with primary bile acid biosynthesis.
- Type of samples: Plasma, serum, cell, tissue, etc.
- Quantity: At least 200 μl liquid, 1 x 10⁷ cells, or 200 mg tissue.
- Storage and Shipping: Samples should be stored at -80℃ and shipped with dry ice.
Primary bile acids, including cholic acid and chenodeoxycholic acid, are synthesized in the liver from cholesterol through a series of enzymatic reactions known as primary bile acid biosynthesis. This complex process involves several key enzymes, such as cholesterol 7α-hydroxylase (CYP7A1), which catalyzes the initial and rate-limiting step of converting cholesterol into 7α-hydroxycholesterol. Subsequent reactions involve multiple enzymes, including sterol 27-hydroxylase (CYP27A1), 12α-hydroxylase (CYP8B1), and others, leading to the formation of primary bile acids. These acids play a crucial role in the digestion and absorption of dietary fats and fat-soluble vitamins and act as signaling molecules in various metabolic pathways. Abnormalities in primary bile acid biosynthesis can lead to serious diseases such as progressive familial intrahepatic cholestasis (PFIC) and various metabolic disorders. MtoZ Biolabs utilizes advanced high-throughput metabolomics techniques, coupled with the power of mass spectrometry, to provide a comprehensive analysis service for primary bile acid biosynthesis.
Services at MtoZ Biolabs
At MtoZ Biolabs, we utilize HPLC-MS/MS to perform primary bile acid biosynthesis and metabolism analysis. This technique is used to separate and quantify the primary bile acids, providing detailed insights into the status of primary bile acid biosynthesis in your samples. Our skilled analysts can assist in interpreting the complex data generated from HPLC-MS/MS, providing valuable insights for your research or clinical studies.
Analysis Workflow
Service Advantages
Applications
Sample Submission Requirements
Deliverables
1. Experimental Procedures
2. Relevant Mass Spectrometry Parameters
3. Detailed Information on Primary Bile Acid Biosynthesis Analysis
4. Mass Spectrometry Images
5. Raw Data
Experience the MtoZ Biolabs difference today. Our primary bile acid biosynthesis analysis service is designed to provide you with the most reliable and actionable results. Contact us for more information.
How to order?
