Principle of Multiple Reaction Monitoring

Multiple Reaction Monitoring (MRM) is a sensitive analytical method based on mass spectrometry, widely used for the detection and quantification of biomarkers in fields such as proteomics, drug metabolism, and environmental monitoring.

 

Basic Principles of MRM

1. Selective Ion Monitoring

Multiple reaction monitoring centers on selectively monitoring target compounds. Using tandem mass spectrometry (MS/MS), specific precursor ions are selected and fragmented in the collision chamber to yield characteristic product ions, enhancing selectivity and sensitivity.

 

2. Ionization

The first step in MRM analysis involves converting target molecules into ions. Common methods include Electrospray Ionization (ESI) and Matrix-Assisted Laser Desorption Ionization (MALDI). Each ionization technique is suited for different sample types, making the choice of method critical for successful MRM.

 

3. Sequential Mass Spectrometric Analysis

Initially, precursor ions are selected, and their mass-to-charge ratio (m/z) is determined. Then, specific product ions are detected. By configuring mass spectrometry parameters, MRM can monitor multiple reactions in real time, significantly enhancing analytical efficiency and accuracy.

 

4. Quantitative Analysis

Multiple reaction monitoring also excels in quantitative analysis. This is achieved by comparing the relative intensities of target ions to internal standards, which helps eliminate variations during sample preparation and instrumental analysis, improving result accuracy.

 

5. Data Acquisition and Analysis

Data acquisition in MRM is critical. The mass spectrometer collects data on the intensities of selected ions over time, allowing for the construction of concentration versus response curves, which can be statistically analyzed to validate result reliability.

 

MtoZ Biolabs provides integrate Multiple Reaction Monitoring service.