PRM LC-MS Targeted Proteomics
Proteomics is an essential approach in biological research for exploring protein functions and structures. Recently, PRM (Parallel Reaction Monitoring) coupled with LCMS (Liquid Chromatography-Mass Spectrometry) has emerged as a highly precise method for targeted protein analysis, enabling quantitative measurements of specific proteins within the proteome.
PRM LCMS is a targeted quantification technique employing liquid chromatography-mass spectrometry. It is characterized by its high precision and reproducibility. PRM functions through selective ion monitoring using the mass spectrometer's full scan capability to detect specific protein ions.
Mechanism of PRM LCMS
1. Selective Ion Monitoring
The process starts with a predefined list of target protein ions. The mass spectrometer is programmed to focus solely on these ions, disregarding others.
2. Ion Fragmentation
Target ions undergo fragmentation within the mass spectrometer, resulting in a range of fragment ions.
3. Fragment Ion Detection
These fragment ions are subsequently detected by the mass spectrometer, producing a mass spectrum.
4. Data Analysis
By comparing experimental results with theoretical data, researchers can accurately quantify the concentration of target proteins.
PRM LCMS Applications
Thanks to its precision and reproducibility, PRM LCMS is widely applied across various research domains. It is instrumental in biomarker discovery, elucidating protein expression patterns, investigating protein structures and functions, and monitoring protein metabolic processes. Additionally, it is extensively utilized in drug development to analyze drug distribution, metabolism, and excretion.
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
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