Protein β-Hydroxybutyrylation Analysis

Post-translational modifications (PTMs) refer to chemical alterations that occur during or after protein synthesis and play critical roles in cellular processes such as differentiation, degradation, signal transduction, gene expression regulation, and protein interactions. β-Hydroxybutyrylation (Kbhb), a novel PTM, occurs under conditions of β-hydroxybutyrate (BHB) accumulation and involves the covalent attachment of a β-hydroxybutyryl group to lysine residues. This modification plays a key role in regulating energy metabolism, gene expression, and stress response. Investigating β-hydroxybutyrylation through target protein β-hydroxybutyrylation analysis and β-hydroxybutyrylation proteomics provides valuable insights into metabolic diseases and other pathological conditions, while contributing to the development of novel therapeutic strategies.

 

Services at MtoZ Biolabs

MtoZ Biolabs utilizes Thermo Fisher's Q Exactive HF, Orbitrap Fusion, and Orbitrap Fusion Lumos mass spectrometry platforms, in combination with Nano-LC, to offer a comprehensive solution for protein β-hydroxybutyrylation analysis, which include target protein β-hydroxybutyrylation analysis and β-hydroxybutyrylation proteomics. Simply provide us with your experimental objectives and sample materials, and we will manage the entire workflow, delivering detailed identification and quantification of β-hydroxybutyrylation modifications on specific proteins and a panoramic view of β-hydroxybutyrylation modifications across various samples, uncovering extensive β-hydroxybutyrylation patterns. If you are interested in our protein β-hydroxybutyrylation analysis service, please feel free to contact us. We are dedicated to providing you with the best service.

 

Analysis Workflow

 



 

Service Advantages

1. Advance Analysis Platform: Mtoz Biolabs established an advanced protein β-hydroxybutyrylation analysis platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.

 

3. High-Data-Quality: Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein β-hydroxybutyrylation analysis data providing clients with acomprehensive data report.

 

4. Comprehensive Analysis: Both target protein β-hydroxybutyrylation analysis and  β-hydroxybutyrylation proteomics can be conducted in MtoZ Biolabs.

 

Sample Submission Requirements

 



1. If you are providing tissue samples, please ship them to us on dry ice.

 

2. If you are providing protein samples, standard tissue or cell lysis buffers can be used during protein extraction.

 

3. Sample transportation: Please ensure that samples are shipped with sufficient dry ice and choose the fastest possible shipping way to minimize the risk of sample degradation during transit.

 

4. Before proceeding with the formal experiment, we will test the samples you provide, and the formal analysis will begin once the samples pass the quality check.

 

Deliverables

1. Comprehensive Experimental Details

2. Materials, Instruments, and Methods

3. The Detailed Information of Protein β-Hydroxybutyrylation Analysis

4. Mass Spectrometry Image

5. Bioinformatics Analysis

6. Raw Data