Protein De Novo Sequencing Service

In the ever-evolving landscape of proteomics, the ability to accurately determine amino acid sequences without prior knowledge of the protein structure is paramount for elucidating their functions, interactions, and roles in various biological processes. MtoZ Biolabs is at the forefront of this scientific frontier, offering comprehensive Protein De Novo Sequencing Services tailored to meet the intricate demands of contemporary biological research and therapeutic development. Utilizing the high-resolution mass spectrometer and leveraging extensive bioinformatics expertise, we provide rapid and accurate analysis of protein primary structures.

 

Traditional protein sequencing methods, whether utilizing MALDI-TOF mass spectrometers or nanoLC-MS/MS platforms, rely on sequence databases that contain the proteins to be identified. These methods involve comparing the molecular weight data obtained from mass spectrometry with the data from the database to sequence and identify proteins. However, certain categories of proteins are often absent from existing databases, such as novel, previously unreported proteins, proteins isolated from new species, or antibodies drugs.

 

In these situations, de novo sequencing is the only method for deciphering the protein sequences. Protein de novo sequencing involves directly inferring the amino acid sequence from experimental mass spectrometry data without depending on existing sequence databases. This approach reconstructs the protein sequence solely based on the observed fragmentation patterns in MS data, making it indispensable for identifying novel proteins, characterizing unexpected post-translational modifications (PTMs), and studying proteins from organisms with unsequenced genomes.

 

Analysis Workflow

 



 

Service Advantages

1. 100% Sequence Coverage

In the protein sequencing process, we employ five different proteases for enzymatic digestion. The complementarity of the resulting peptide fragments allows us to achieve full protein sequence assembly, ensuring 100% sequence coverage.

 

2. Advance Analysis Platform

MtoZ Biolabs established an advanced protein de novo sequencing service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

3. Innovative Data Processing Algorithm

Building on the latest research and utilizing the PEAKS Mass Spectrometry Software for database-independent protein sequence analysis, we have developed our own computational algorithm for processing raw data. This ensures accurate protein sequence identification without the loss of any relevant data.

 

4. Rigorous Sequence Prediction and Validation

We first perform prediction on the sequencing results. Then, we create a new database from the determined sequences and employ reverse sequence conformation mode for data verification, ensuring that only fully verified and matched results are delivered to our clients.

 

5. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrate all protein de novo sequencing data providing clients with a comprehensive data report.

 

6. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

Applications

1. Sequence Analysis of Protein/Peptide, of which Databases are Unavailable

2. PTMs Analysis of Protein/Peptide, of which Databases are Unavailable

3. Sequence Analysis of CDR Region or Full-Length of Antibodies

4. Sequence Analysis of Commercially Engineered Proteins and Enzymes

 

FAQ

Q1: What types of samples are accepted for de novo sequencing?

We accept recombinant proteins, peptides, fluorescently labeled antibodies, monoclonal antibodies, bispecific antibodies, multispecific antibodies, cross-linked antibodies, and other types of proteins. If you have unique samples, our experts can provide tailored solutions to accommodate your research needs.

 

Q2: Will sample post-translational modifications (PTMs) affect the result of sequencing?

Post-translational modifications do not affect the sequencing results themselves. During data analysis, we account for potential PTMs, ensuring that these modifications are accurately identified and incorporated without compromising the integrity of the protein sequence determination.

 

Deliverables

1. Experimental Procedures

2. Relevant Mass Spectrometry Parameters

3. Detailed Information on Protein Sequencing

4. Mass Spectrometry Images

5. Raw Data

 

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. Our ultimate aim is to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. At MtoZ Biolabs, we are committed to providing high-quality, reliable, and personalized analysis services. If you are interested in our protein de novo sequencing service, please feel free to contact us.