Protein Full-Length Sequencing Service at MtoZ Biolabs

Protein full-length sequencing ensures protein integrity and verifies accurate translation, providing critical molecular insights for drug development. MtoZ Biolabs, leveraging advanced mass spectrometry technologies, delivers a comprehensive protein full-length sequencing service tailored to biopharmaceuticals, antibody development, vaccine research, and more.

 

Analysis Workflow

MtoZ Biolabs' protein full-length sequencing service comprises three primary steps:

 

1. Protein Digestion

In the initial step, proteins are enzymatically digested into smaller peptide fragments. Traditional sequencing methods often rely on trypsin digestion, which typically results in about 60% coverage. To enhance sequence coverage, MtoZ Biolabs incorporates up to six protease combinations, ensuring more comprehensive detection of peptide fragments.

 

2. LC-MS/MS Analysis

Following peptide separation using liquid chromatography (LC), tandem mass spectrometry (MS/MS) is employed to analyze the mass-to-charge ratios of the peptides, allowing for precise identification of their sequences.

 

3. Data Analysis

Using advanced algorithms, the MS/MS data is processed to reconstruct the full protein sequence while identifying post-translational modifications (PTMs) and other chemical modifications.

 

Applications

1. Determination of Full-Length Sequence of Proteins/Peptides/Antibodies/Vaccines

2. Analysis of PTMs & Chemical Modifications on Protein

3. Verification of the Correct Translation of Recombinant Proteins

 

Deliverables

1. Experimental Procedures

2. Parameters for Liquid Chromatography and Mass Spectrometry

3. MS Raw Data Files

4. Peptide Identification and Signal Intensity

5. Protein Identification and Signal Intensity